推荐产品
形式
tablet
质量水平
适用性
suitable for identification of lac+ bacterial colonies
储存温度
−20°C
SMILES字符串
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key
OPIFSICVWOWJMJ-AEOCFKNESA-N
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一般描述
5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷，通常称为 X-Gal，是 β-半乳糖苷酶的组织化学底物。
应用
5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷已用于制备 X-Gal 储备液，并将其添加到 LB 琼脂平板中，用于蓝白色筛选转化子。
X-Gal是β-半乳糖苷酶的发色底物，可产生丰富的蓝色，与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。
生化/生理作用
X-gal 通常用于转基因活性的原位检测。
数量
每片含 5 mg 底物
重悬
通过将 X-Gal 溶解在二甲基甲酰胺中制备储备液（100 mg/ml），应将其储存在 −20°C 并避光。无需灭菌。
相关产品
产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies
Fields C, et al.
Nature Protocols, 8(6), 1125-1125 (2013)
Analysis of Transgene Expression
Transactions of the American Clinical and Climatological Association, 543-564 (2014)
Yibing Wang et al.
PloS one, 8(3), e59195-e59195 (2013-03-26)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a
Amy McCurley et al.
Nature medicine, 18(9), 1429-1433 (2012-08-28)
Hypertension is a cardiovascular risk factor present in over two-thirds of people over age 60 in North America; elevated blood pressure correlates with increased risk of heart attack, stroke and progression to heart and kidney failure. Current therapies are insufficient
Florian Weinberger et al.
Circulation research, 110(10), 1303-1310 (2012-03-20)
The transcription factor Islet-1 is a marker of cardiovascular progenitors during embryogenesis. The isolation of Islet-1-positive (Islet-1(+)) cells from early postnatal hearts suggested that Islet-1 also marks cardiac progenitors in adult life. We investigated the distribution and identity of Islet-1(+)
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