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Merck
CN

B6024

Sigma-Aldrich

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

tablet, suitable for identification of lac+ bacterial colonies

别名:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

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About This Item

经验公式(希尔记法):
C14H15BrClNO6
CAS号:
分子量:
408.63
Beilstein:
1402009
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

产品名称

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, tablet

表单

tablet

质量水平

适用性

suitable for identification of lac+ bacterial colonies

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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一般描述

5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷,通常称为 X-Gal,是 β-半乳糖苷酶的组织化学底物。

应用

5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷已用于制备 X-Gal 储备液,并将其添加到 LB 琼脂平板中,用于蓝白色筛选转化子。
X-Gal是β-半乳糖苷酶的发色底物,可产生丰富的蓝色,与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

生化/生理作用

X-gal 通常用于转基因活性的原位检测。

数量

每片含 5 mg 底物

重悬

通过将 X-Gal 溶解在二甲基甲酰胺中制备储备液(100 mg/ml),应将其储存在 −20°C 并避光。无需灭菌。

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Analysis of Transgene Expression
Transactions of the American Clinical and Climatological Association, 543-564 (2014)
Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies
Fields C, et al.
Nature Protocols, 8(6), 1125-1125 (2013)
Hiroyuki Kanazawa et al.
PloS one, 6(4), e19195-e19195 (2011-05-12)
Ischemia-reperfusion (I/R) injury associated with living donor liver transplantation impairs liver graft regeneration. Mesenchymal stem cells (MSCs) are potential cell therapeutic targets for liver disease. In this study, we demonstrate the impact of MSCs against hepatic I/R injury and hepatectomy.
Enzo R Porrello et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(1), 187-192 (2012-12-19)
We recently identified a brief time period during postnatal development when the mammalian heart retains significant regenerative potential after amputation of the ventricular apex. However, one major unresolved question is whether the neonatal mouse heart can also regenerate in response
Khanh-Van Tran et al.
Cell metabolism, 15(2), 222-229 (2012-02-14)
Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (ECs) of classic white and brown fat

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