B5144
菠萝蛋白酶 来源于菠萝的茎
≥4 units/mg protein, (chromatography purified)
产品名称
菠萝蛋白酶 来源于菠萝的茎, ≥4 units/mg protein, (chromatography purified)
form
lyophilized powder
specific activity
(chromatography purified)
≥4 units/mg protein
composition
Protein, 30-50% biuret
solubility
H2O: soluble 1.0 mg/mL, clear, colorless
storage temp.
−20°C
Quality Level
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Preparation Note
色谱纯化。溶于 1 mg/mL 浓度的水中，形成澄清、无色溶液。
色谱纯化产品
Application
来自菠萝茎的菠萝蛋白酶已被用作十二烷基硫酸钠（SDS）/聚丙烯酰胺凝胶电泳（PAGE）酶谱中的阳性对照。它还被用作测定蛋白水解活性的偶氮酪蛋白底物。
菠萝蛋白酶可用于抑制促炎前列腺素的生物合成。可用于降低凝血效率。来自菠萝茎的菠萝蛋白酶已被用于制备蜜蜂采集的花粉的酶水解产物。
Biochem/physiol Actions
菠萝蛋白酶是一种半胱氨酸内肽酶，对蛋白质的裂解具有广泛的特异性。菠萝蛋白酶可能来自干或水果。产品 P3169（pGlu-Phe-Leu-P-硝基苯胺）可用作菠萝蛋白酶含量测定的显色底物。
Other Notes
25 °C 下 pH 4.6 下，1 个单位每分钟将从 N-α-Z-L-赖氨酸对硝基苯酯中释放 1.0 μmol 对硝基苯酚。
Physical form
含甘露醇和磷酸钾缓冲盐的冻干粉
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
此项目有
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Nora Luniak et al.
Biotechnology progress, 33(1), 54-65 (2016-11-20)
Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion of host proteins. On the other hand, proteases are broadly used in industrial applications and resemble promising drug candidates. Bromelain is an herbal
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a
Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Larisa V Kordyukova et al.
Membranes, 11(10) (2021-10-23)
Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral
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