推荐产品
应用
来自菠萝茎的菠萝蛋白酶已被用作十二烷基硫酸钠（SDS）/聚丙烯酰胺凝胶电泳（PAGE）酶谱中的阳性对照。它还被用作测定蛋白水解活性的偶氮酪蛋白底物。
菠萝蛋白酶可用于抑制促炎前列腺素的生物合成。可用于降低凝血效率。来自菠萝茎的菠萝蛋白酶已被用于制备蜜蜂采集的花粉的酶水解产物。
生化/生理作用
菠萝蛋白酶是一种半胱氨酸内肽酶，对蛋白质的裂解具有广泛的特异性。菠萝蛋白酶可能来自干或水果。产品 P3169（pGlu-Phe-Leu-P-硝基苯胺）可用作菠萝蛋白酶含量测定的显色底物。
单位定义
25 °C 下 pH 4.6 下，1 个单位每分钟将从 N-α-Z-L-赖氨酸对硝基苯酯中释放 1.0 μmol 对硝基苯酚。
外形
含甘露醇和磷酸钾缓冲盐的冻干粉
制备说明
色谱纯化。溶于 1 mg/mL 浓度的水中，形成澄清、无色溶液。
色谱纯化产品
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a
Basir Ahmad et al.
Biopolymers, 81(5), 350-359 (2005-12-14)
The effect of low, medium, and high molecular weight poly(ethylene glycol) (e.g., PEG-400, -6000, and -20,000) on the structure of the acid unfolded state of unmodified stem bromelain (SB) obtained at pH 2.0 has been studied by various spectroscopic methods.
V Grabovac et al.
International journal of pharmaceutics, 326(1-2), 153-159 (2006-09-05)
The aim of this study was to investigate the influence of the proteolytic enzyme bromelain on the permeation of heparin across the gastrointestinal epithelial barrier. Stability of the complex and effect of heparin on the enzymatic activity of bromelain was
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