B4554
N，N-双（2-羟乙基）-2-氨基乙磺酸
BioPerformance Certified, suitable for cell culture, ≥99.0%
别名:
2-(二乙醇氨基)乙磺酸, N,N-双(2-羟乙基)-2-氨基乙磺酸
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关于此项目
经验公式（希尔记法）:
C6H15NO5S
化学文摘社编号:
分子量:
213.25
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
233-465-5
Beilstein/REAXYS Number:
1781572
MDL number:
assay
≥99.0%
InChI key
AJTVSSFTXWNIRG-UHFFFAOYSA-N
InChI
1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
SMILES string
OCCN(CCO)CCS(O)(=O)=O
grade
BioPerformance Certified
form
crystalline
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin and bioburden, tested
pH
2.5-5.0 (25 °C, 213.3 g/L)
useful pH range
6.4-7.8
pKa (25 °C)
7.1
solubility
water: 0.5 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
foreign activity
DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
storage temp.
room temp
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General description
N-N-双(2-羟乙基)-2-氨基乙磺酸（BES）是一种两性离子缓冲液，常用于生物化学和分子生物学研究中。BES缓冲液可用作二级pH标准物质，能使溶液保持在稳定的pH范围内，特别是在6.2-7.6范围内。一项研究表明，在细菌内毒素检查中使用这种缓冲液可以最大限度地减少干扰因素的影响。BES还可用作磷酸钙介导质粒DNA转染真核细胞的缓冲溶液。
Application
BES缓冲液可用于：
- 作为体外分析胸腺细胞凋亡时培养胸腺细胞用的opti-minimal essential medium（MEM）培养基的补充剂
- 作为腺相关病毒（AAV）载体制备、提取和纯化用的硼酸盐缓冲液（BBS）的组分
- 作为精液采集和冷冻保存用的稀释剂之一的组分
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Effects of amino acid sequence, buffers, and ionic strength on the rate and mechanism of deamidation of asparagine residues in small peptides
Tyler-Cross R and Schirch V
The Journal of Biological Chemistry, 266(33) , 22549-22556 (1991)
Saline and buffers minimize the action of interfering factors in the bacterial endotoxins test
Fujita Y, et al.
Analytical Biochemistry, 409(1), 46-53 (2011)
Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs
Sambrook J and Russell D W
CSH Protocols, 2006(1) (2006)
Standard buffer of N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (Bes) for use in the physiological pH range 6.6 to 7.4
Roy R N, et al.
Analytical Chemistry, 47(8) (1975)
Cryopreservation of Korean Oge chicken semen using N-methylacetamide
Hong Jo L, et al.
Cryo letters, 33(6), 427-434 (2012)
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