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Merck
CN

B4287

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.25

生物来源

bovine

质量水平

等级

for molecular biology

产品线

BioReagent

方案

≥98%

表单

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

poly bottle of

来源

USA origin

技术

ELISA: suitable
electrophoresis: suitable
western blot: suitable

pH值(酸碱度)

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

Protease free

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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应用

A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特点和优势

  • Protease free
  • Suitable for blocking non-specific protein binding sites in hybridizations
  • Heat shock fractionated

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

新产品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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访问文档库

Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Gautam Adhikary et al.
Carcinogenesis, 36(7), 800-810 (2015-05-15)
Polycomb group proteins, including Ezh2, are important candidate stem cell maintenance proteins in epidermal squamous cell carcinoma. We previously showed that epidermal cancer stem cells (ECS cells) represent a minority of cells in tumors, are highly enriched in Ezh2 and
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Marcos V A S Navarro et al.
PLoS biology, 9(2), e1000588-e1000588 (2011-02-10)
The bacterial second messenger bis-(3'-5') cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which in Pseudomonas fluorescens requires the transmembrane receptor LapD. LapD exhibits a
Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.

实验方案

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

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