应用
BCIP®/NBT-紫色液体膜底物系统已被用作碱性磷酸酶的底物
- 用于通过免疫印迹法检测二抗
- 用于ELISPOT测定分析
- 用于斑点印迹分析
- 用于免疫印迹分析，以可视化硝酸纤维素膜上的蛋白条带
生化/生理作用
5-溴-4-氯-3-吲哚基磷酸酯 (BCIP)/硝基蓝四唑（NBT）/硝基蓝四唑鎓(NBT)是用于检测碱性磷酸酶(AP)的显色底物系统。AP水解BCIP形成中间体无色靛蓝化合物，并进一步被NBT氧化形成5,5′-二溴-4,4′-二氯靛蓝(BCI)。其中，NBT被还原为不溶的紫色双甲双胍(DF)，形成NBT-DF。NBT-DF和BCI的混合物形成深紫色沉淀。AP可水解多种BCIP分子，因此成为了一种高灵敏度方法。
注意
在2-8 °C稳定至少一年。
外形
即用型。
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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