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经验公式(希尔记法):
C19H22N6O4 · HCl
化学文摘社编号:
分子量:
434.88
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
244-505-6
MDL number:
Beilstein/REAXYS Number:
4081878
产品名称
Nα-苯甲酰-L-精氨酸-4-硝基苯胺 盐酸盐, ≥98% (HPLC)
InChI
1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1
InChI key
DEOKFPFLXFNAON-NTISSMGPSA-N
SMILES string
Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O
assay
≥98% (HPLC)
form
powder
solubility
acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow
storage temp.
−20°C
Quality Level
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General description
胰蛋白酶和其他蛋白水解酶的显色底物。
Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.
Application
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:
- to measure papain activity in spectrophotometric amidase assay
- to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
- for trypsin
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm
Zhou Y, et al.
Protein Expression and Purification, 111, 18- 22 (2015)
A chemical approach for detecting sulfenic acid-modified proteins in living cells
Reddie KG, et al.
Molecular Biosystems, 4(6), 521- 531 (2008)
Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
He YY, et al.
Peptides, 29(10), 1692- 1699 (2008)
Daniele O B Sousa et al.
Nutrients, 7(7), 6038-6054 (2015-07-25)
The biochemical and nutritional attributes of two soybean (Glycine max (L.) Merr.) cultivars, one susceptible (Seridó) and the other resistant (Seridó-RCH) to stem canker, were examined to assess whether the resistance to pathogens was related to levels of antinutritional and/or
Raquel O Rocha et al.
Journal of agricultural and food chemistry, 63(22), 5335-5343 (2015-06-03)
Several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. This study focused on the proteins released in the exudates of soybean
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