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Merck
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文件

安全信息

B2787

Sigma-Aldrich

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

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别名:
protein markers, protein molecular weight markers, protein standards
UNSPSC代码:
12352200
eCl@ss:
34058011
NACRES:
NA.25

形式

lyophilized powder

质量水平

200

用途

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

分子量

6,500-180,000 Da

包装

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

储存温度

−20°C

相关类别

应用

对于 SDS-PAGE 和蛋白转移，生物素化分子量标准品混合物含有蛋白，而这些蛋白已与生物素结合，从而用作估算带印迹蛋白分子量的 SDS-PAGE 标准品。使用链霉亲和素-过氧化物酶（编号： S2438 ）和4-氯-1-萘酚（编号：C8890）等显色剂实现显谱，从而避免为一般蛋白染色切出单独通道，且这可与免疫染色程序同时进行。这些标准品可用于 Laemmli 或 Webe和Osborn SDS-PAGE程序。

组分

本品含有：
  • α 2 -巨球蛋白（马血清）
  • β-半乳糖苷酶（ 大肠杆菌 ）
  • 磷酸化酶 b（兔肌肉）
  • 过氧化氢酶（牛肝）
  • 乙醇脱氢酶（酵母）
  • 碳酸酐酶（牛红细胞）
  • 胰蛋白酶抑制剂（大豆）
  • 溶菌酶（鸡蛋）
  • 抑肽酶（牛肺）
每小瓶约含 33% 蛋白质、33%NaCl 和 33%蔗糖。

其他说明

含有 9 种生素蛋白（总计约 0.1 mg）。

重悬

用 1 mL 1x 样品缓冲液（产品目录号：S3401）重建每个小瓶，并在100°C培养2-3分钟。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. The data sheets for Products SDS7B2 and B2787 do not provide a specific recommendation on how long the solutions of these molecular weight marker mixtures can be kept frozen at -20°C.  What information can you provide?

    Our R&D group recommends that after reconstitution, the solutions should be divided into aliquots and immediately frozen; avoid repeated freeze / thaw cycles. The aliquots should last at least a year at -20°C. Storage at -80°C would even be better, if it is convenient.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)

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