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安全信息

B2661

Sigma-Aldrich

抗-fd 噬菌体 − 生物素 兔抗

~3.5 mg/mL, IgG fraction of antiserum

别名:

抗-fd 噬菌体

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

rabbit

质量水平

200

偶联物

biotin conjugate

抗体形式

IgG fraction of antiserum

抗体产品类型

primary antibodies

克隆

polyclonal

浓度

~3.5 mg/mL

技术

indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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特异性

该抗体与 fd 噬菌体或 M13 噬菌体的噬菌体外壳蛋白特异性结合，因此当直接包被在多孔板上或作为特异性捕获 fd 或 M13 噬菌体的一级检测抗体时，可作为捕获抗体发挥作用。该抗体可用于快速分选大型噬菌体展示文库（抗体、肽等），所述表达蛋白与丝状噬菌体的基因 III 或基因 VIII 蛋白融合。该抗体可作为“噬菌体 ELISA”的检测试剂，为重组噬菌体的检测提供灵敏、特异的活性。

免疫原

fd 噬菌体

应用

兔抗 fd 噬菌体-生物素抗体可用于测定免疫球蛋白含量（磁珠免疫测定法）

外形

以 0.01 M 磷酸盐缓冲液（pH 7.4，含有 15mM 叠氮化钠）溶液提供

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000100745
0000100745
0000088047
018M4884
077M4896

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. The description states Product B2661, Anti-fd Bacteriophage, may be used for staining DNA, chromosomes and nuclei, and for fluorescence microscopy or flow cytometry.  Are there other uses for the product?

    The product has also been used for the detection of apoptosis, applied as a label to live cells, as a component of BrdU cell cycle investigation.

  4. What is the expected solution stability for aqueous solutions of Product B2661, Anti-fd Bacteriophage?

    Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.

  5. Which is more cell permeable, H33342 or H33258?

    H33342 has been found to be about 10 times more cell-permeable than H33258. See Science, 282(5387), 296-298, (1998).

  6. What is the specificity for H33342?

    The bisBenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators.  Reference: Loontiens, F. G., et al., Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)] and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry, 29, p. 9029 (1990).

  7. When using Product B2661, Anti-fd Bacteriophage, which is the preferred Hoechst stain for chromosomes?

    Hoechst No. 33258 and not Hoechst No. 33342 is the preferable dye for chromosomal staining.

  8. What is the pH of Product B2661, Anti-fd Bacteriophage, in solution?

    There is no pH test specification for product B2261 on a lot-to-lot basis.  A one-time measurement of the pH of a 2% solution was 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.

  9. Will chromosomes that are dividing or replicating stain with Product B2661, Anti-fd Bacteriophage?

    No; chromosomes that are dividing or replicating will not stain with this dye.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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