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Merck
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安全信息

B0287

Sigma-Aldrich

单克隆抗 FITC-生物素 小鼠抗

clone FL-D6, purified immunoglobulin, buffered aqueous solution

别名:

单克隆抗-FITC

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.43

生物来源

mouse

质量水平

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

FL-D6, monoclonal

表单

buffered aqueous solution

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特异性

该抗体可与游离的和结合的异硫氰酸荧光素(FITC)反应。

免疫原

FITC-BSA偶联物

应用

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
  • for amplification of signal in immunofluorescence assays
  • in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
  • enzyme linked immunosorbent assay (ELISA)

生化/生理作用

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.
Liu, et al.
Cardiovascular Research, 119, 1046-1061 (2023)
Flow-based microimmunoassay
Hayes MA, et al.
Analytical Chemistry, 73(24), 5896-5902 (2001)
Kazuyuki Kasahara et al.
Cell host & microbe, 31(6), 1038-1053 (2023-06-07)
The microbes and microbial pathways that influence host inflammatory disease progression remain largely undefined. Here, we show that variation in atherosclerosis burden is partially driven by gut microbiota and is associated with circulating levels of uric acid (UA) in mice
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Peter Stachon et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(10), 2237-2245 (2014-08-12)
Nucleotides such as ATP, ADP, UTP, and UDP serve as proinflammatory danger signals via purinergic receptors on their release to the extracellular space by activated or dying cells. UDP binds to the purinergic receptor Y6 (P2Y6) and propagates vascular inflammation

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