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一般描述
GreenUniversal Detection Reagent is formulated with green fluorescent protein (GFP),which is a part of the split fluorescent protein (FP) system that may be usedto quantify the expression level of a tagged protein of interest (POI).
应用
The S1-10 Universal Detection Reagent is provided as a ready-to-use solution for use with the In Vitro "Fold ′n′ Glow" Solubility Assay Kits:
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Stéphanie Cabantous et al.
Nature biotechnology, 23(1), 102-107 (2004-12-08)
Existing protein tagging and detection methods are powerful but have drawbacks. Split protein tags can perturb protein solubility or may not work in living cells. Green fluorescent protein (GFP) fusions can misfold or exhibit altered processing. Fluorogenic biarsenical FLaSH or
G S Waldo et al.
Nature biotechnology, 17(7), 691-695 (1999-07-15)
Formation of the chromophore of green fluorescent protein (GFP) depends on the correct folding of the protein. We constructed a "folding reporter" vector, in which a test protein is expressed as an N-terminal fusion with GFP. Using a test panel
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