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Merck
CN

AP0100

Sigma-Aldrich

碱性磷酸酶二乙醇胺活性检测试剂盒

sufficient for 100 tests

别名:

ALP Activity Kit

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.26

用途

sufficient for 100 tests

质量水平

储存温度

2-8°C

相关类别

一般描述

碱性磷酸酶二乙醇胺检测试剂盒提供了一种检测碱性磷酸酶活性的即用型试剂。这种简单的检测分析碱性磷酸酶活性的方法使用了p-磷酸对硝基苯酯(pNPP)作为底物。

应用

碱性磷酸酶活性二乙醇胺法检测试剂盒已被用于:
  • 检测血清和成骨细胞中碱性磷酸酶(ALP)的水平
  • 监测小鼠成肌细胞C2C12细胞在成骨细胞分化过程中的ALP水平
  • 测定肺癌骨转移模型中的ALP活性
  • 测定结肠组织匀浆中的ALP水平

适用性

适用于多种样品中碱性磷酸酶活性的测定。

原理

碱性磷酸酶将pNPP水解为对硝基苯酚和无机磷酸盐。在37°C下孵育碱性磷酸酶样品和底物期间,通过监测405 nm处吸光度的增加来追踪反应。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powder化学品安全说明书

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg protein化学品安全说明书

象形图

Health hazardCorrosion

警示用语:

Danger

危险分类

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

靶器官

Kidney,Liver,Blood

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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