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安全信息

AC-6000

Sigma-Aldrich

HighPrep PCR Clean-up System

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About This Item

UNSPSC代码:
41105500

表单

liquid

包装

pkg of 250 mL (AC-60250 (HighPrep PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep PCR (500 mL)))

储存条件

do not freeze

储存温度

2-8°C

一般描述

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

应用

HighPrep PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up
  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

特点和优势

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

储存及稳定性

14 months when stored as directed

法律信息

HighPrep is a trademark of MagBio Genomics, Inc.

免责声明

For Research Use Only. Not for Use in Diagnostic Procedures.

象形图

Health hazard

警示用语:

Warning

危险声明

危险分类

Repr. 2

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Madhu Choudhary et al.
3 Biotech, 8(7), 304-304 (2018-07-14)
In Indo-Gangetic plains (IGP) of India, natural resources (soil, water, and environment) are degrading under the conventional-till (CT)-based management practices in rice-wheat cropping system. A long-term field experiment was conducted to understand the soil bacterial diversity and abundance under different
Deep sequencing of near full-length HIV-1 genomes from plasma identifies circulating subtype C and infrequent occurrence of AC recombinant form in Southern India}
Alampalli SV, et al.
PLoS ONE, 17 (2017)
Soil bacterial diversity under conservation agriculture-based cereal systems in Indo-Gangetic Plains
Choudhary M, et al.
3 Biotech, 8, 304-304 (2018)
N Salvetat et al.
Journal of neurovirology, 25(6), 825-836 (2019-07-25)
Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy with IFN-α and ribavirin. It has recently been shown that changes in A-to-I RNA editing rates are associated with various pathologies
Vaishali Sah et al.
Gene, 731, 144356-144356 (2020-01-15)
The genetic basis of differential host immune response vis-à-vis transcriptome profile was explored in PBMCs of indigenous (Ghurrah) and crossbred pigs after classical swine fever vaccination and in monocyte derived macrophages (MDMs) challenged with virulent classical swine fever (CSF) virus.

实验方案

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.

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