form
liquid
packaging
pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL))), pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL))), pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL))), pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))
storage condition
do not freeze
storage temp.
2-8°C
General description
Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
Application
HighPrep™ PCR Clean-up System may be used in:
- Post-PCR and post-enzymatic reaction clean-up
- NGS library preparation
- Amplicon size selection for NGS
- Microarrays
- PCR
- Sanger sequencing
- Restriction enzyme digestions, adapter ligations
- Cloning
Features and Benefits
- High recovery of >100 bp amplicons
- Adaptable to high throughput liquid handling systems
- Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
- No centrifugation or filtration leads to higher and purer yields
Preparation Note
14 months when stored as directed
Legal Information
HighPrep is a trademark of MagBio Genomics, Inc.
Disclaimer
For Research Use Only. Not for Use in Diagnostic Procedures.
signalword
Warning
hcodes
Hazard Classifications
Repr. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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实验方案
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
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