产品名称
碱性磷酸酶蓝膜底物溶液, sufficient for 400 mL working substrate
form
solution
usage
sufficient for 400 mL working substrate
storage temp.
2-8°C
Quality Level
General description
碱性磷酸酶是一种金属酶，属于外切核苷酸酶家族。存在于胆管、骨、肠、胎盘和肿瘤中。
Application
在与碱性磷酸酶反应之前，BCIP®试剂为无色至淡蓝色，硝基BT试剂为黄色。在膜型测定中，两组分混合物与碱性磷酸酶形成蓝紫色产物。不建议将该底物用于ELISA分析。
碱性磷酸酶蓝膜底物溶液已用于：
- 分析直接碱性磷酸酶（AP）活性
- 用比色系统进行碱性磷酸酶测定
- 分析诱导多能干细胞（iPSC）系LND554SV.3的直接碱性磷酸酶（AP）活性
Physical form
2组分试剂盒足以制备400 mL工作底物。
作为含溴氯吲哚磷酸盐（BCIP®）和硝基蓝四唑（NBT/噻唑蓝/硝基BT）的双组分缓冲碱性磷酸酶底物提供
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Inhibition of alkaline phosphatase: an emerging new drug target
Al-Rashida M and Iqbal J
Mini-Reviews in Medicinal Chemistry, 15(1), 41-51 (2015)
The Short Isoform of NF-YA Belongs to the Embryonic Stem Cell Transcription Factor Circuitry
Dolfini D, et al.
Stem Cells, 30(11), 2450-2459 (2012)
Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
Stem Cell Research, 16(1), 63-66 (2016)
Generation of pig iPS cells: a model for cell therapy
Montserrat N, et al.
Journal of Cardiovascular Translational Research, 4(2), 121-130 (2011)
María Del Carmen Ortuño-Costela et al.
Stem cell research, 23, 188-192 (2017-09-20)
Human iPSC line IISHDOi001-A was generated from fibroblasts of a patient with McArdle disease harbouring the mutation, c.148C>T; p.Arg50Ter, in the PYGM gene. Reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using Sendai virus.
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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