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一般描述
碱性磷酸酶是一种金属酶，属于外切核苷酸酶家族。存在于胆管、骨、肠、胎盘和肿瘤中。
应用
在与碱性磷酸酶反应之前，BCIP®试剂为无色至淡蓝色，硝基BT试剂为黄色。在膜型测定中，两组分混合物与碱性磷酸酶形成蓝紫色产物。不建议将该底物用于ELISA分析。
碱性磷酸酶蓝膜底物溶液已用于：
- 分析直接碱性磷酸酶（AP）活性
- 用比色系统进行碱性磷酸酶测定
- 分析诱导多能干细胞（iPSC）系LND554SV.3的直接碱性磷酸酶（AP）活性
外形
2组分试剂盒足以制备400 mL工作底物。
作为含溴氯吲哚磷酸盐（BCIP®）和硝基蓝四唑（NBT/噻唑蓝/硝基BT）的双组分缓冲碱性磷酸酶底物提供
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Inhibition of alkaline phosphatase: an emerging new drug target
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Human iPSC line, IISHDOi006-A, was obtained from fibroblasts of a patient with Dominant Optic Atrophy (DOA) carrying a heterozygous mutation in the gene ACO2: c.1999G>A; p.Glu667Lys. Reprogramming factors Oct3/4, Sox2, Klf4, and c-Myc were delivered using a non-integrative methodology that
Generation of a human iPSC line from a patient with Leigh syndrome
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We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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