推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 110 kDa (additional band may be observed at 45 kDa)
种属反应性
rat, human
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections
immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg)
microarray: suitable
western blot: 1-2 μg/mL using whole extract of rat brain
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
雄性激素是男性性激素,在与核雄激素受体(AR)结合时发挥生物学和转录作用。人类的雄性激素包括雄烯二酮,睾酮,二氢睾丸激素和脱氢表雄酮。雄激素受体是一种配体诱导型核受体,表达于脑、骨、骨骼肌、生殖器官、毛囊和心血管组织。结合雄激素后,AR被内在化并调节靶基因的转录。此外,AR还具有核外作用,在其中迅速诱导MAPK和蛋白激酶C的信号级联反应。AR的调节导致胚胎发生过程中性器官的不可逆性分化,以及青春期性别特异性特征的发育
抗雄激素受体特异性识别人雄激素受体蛋白(110 kDa)。在45 kDa处可以观察到一个额外的条带。该抗体会与大鼠雄激素受体发生交叉反应。
抗雄激素受体特异性识别人雄激素受体蛋白(110 kDa)。在45 kDa处可以观察到一个额外的条带。该抗体会与大鼠雄激素受体发生交叉反应。
特异性
该抗体会与大鼠雄激素受体发生交叉反应。
免疫原
进化上保守的肽,对应于人雄激素受体的氨基酸1-21。在雌激素、孕激素或糖皮质激素受体中找不到此序列。
应用
兔抗雄激素受体(C19)用于EMSA(电泳迁移率变动分析),该分析在特定DNA序列的分子量中产生超位移,表明雄激素受体的结合。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1篇论文)
蛋白质免疫印迹分析(1篇论文)
抗雄激素受体抗体可用于免疫印迹,使用大鼠全脑提取物,推荐浓度为1-2 μg/mL。对于来自250 μg人MCF7细胞裂解液的免疫沉淀,可以使用5-10 μg的浓度。免疫组化可以在20-40 μg/mL的工作抗体浓度下对石蜡包埋的人前列腺组织切片进行。
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
从最新的版本中选择一种:
分析证书(COA)
Stem cell reports, 18(6), 1355-1370 (2023-05-13)
Both the canonical Wnt and androgen receptor (AR) signaling pathways are important for prostate organogenesis and homeostasis. How they crosstalk to regulate prostate stem cell behaviors remains unclear. Here, we show in lineage-tracing mouse models that although Wnt is essential
Proceedings of the National Academy of Sciences of the United States of America, 98(18), 10344-10349 (2001-08-16)
Attempts to repair myocardial infarcts by transplanting cardiomyocytes or skeletal myoblasts have failed to reconstitute healthy myocardium and coronary vessels integrated structurally and functionally with the remaining viable portion of the ventricular wall. The recently discovered growth and transdifferentiation potential
The Prostate, 79(11), 1326-1337 (2019-06-19)
Serially transplantable patient-derived xenografts (PDXs) are invaluable preclinical models for studying tumor biology and evaluating therapeutic agents. As these models are challenging to establish from prostate cancer specimens, the ability to preserve them through cryopreservation has several advantages for ongoing
Journal of neuroendocrinology, 25(6), 547-559 (2013-02-19)
The seminal vesicles are male accessory sex glands that contribute much of the seminal fluid volume. Previous studies have suggested that the majority of autonomic innervations to the rat seminal vesicles originate from the bilateral major pelvic ganglia. Many preganglionic
The Journal of biological chemistry, 292(33), 13521-13530 (2017-07-07)
NK3 homeobox 1 (Nkx3.1), a transcription factor expressed in the prostate epithelium, is crucial for maintaining prostate cell fate and suppressing tumor initiation. Nkx3.1 is ubiquitously expressed in luminal cells of hormonally intact prostate but, upon androgen deprivation, exclusively labels
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