A9853
抗-雄激素受体 兔抗
affinity isolated antibody, buffered aqueous solution
别名:
抗-AR
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), IP, WB
Citations:
19
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 110 kDa (additional band may be observed at 45 kDa)
species reactivity
rat, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections, immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg), microarray: suitable, western blot: 1-2 μg/mL using whole extract of rat brain
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
雄性激素是男性性激素，在与核雄激素受体（AR）结合时发挥生物学和转录作用。人类的雄性激素包括雄烯二酮，睾酮，二氢睾丸激素和脱氢表雄酮。雄激素受体是一种配体诱导型核受体，表达于脑、骨、骨骼肌、生殖器官、毛囊和心血管组织。结合雄激素后，AR被内在化并调节靶基因的转录。此外，AR还具有核外作用，在其中迅速诱导MAPK和蛋白激酶C的信号级联反应。AR的调节导致胚胎发生过程中性器官的不可逆性分化，以及青春期性别特异性特征的发育
抗雄激素受体特异性识别人雄激素受体蛋白（110 kDa）。在45 kDa处可以观察到一个额外的条带。该抗体会与大鼠雄激素受体发生交叉反应。
抗雄激素受体特异性识别人雄激素受体蛋白（110 kDa）。在45 kDa处可以观察到一个额外的条带。该抗体会与大鼠雄激素受体发生交叉反应。
Immunogen
进化上保守的肽，对应于人雄激素受体的氨基酸1-21。在雌激素、孕激素或糖皮质激素受体中找不到此序列。
Application
兔抗雄激素受体（C19）用于EMSA（电泳迁移率变动分析），该分析在特定DNA序列的分子量中产生超位移，表明雄激素受体的结合。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析（1篇论文）
蛋白质免疫印迹分析（1篇论文）
抗雄激素受体抗体可用于免疫印迹，使用大鼠全脑提取物，推荐浓度为1-2 μg/mL。对于来自250 μg人MCF7细胞裂解液的免疫沉淀，可以使用5-10 μg的浓度。免疫组化可以在20-40 μg/mL的工作抗体浓度下对石蜡包埋的人前列腺组织切片进行。
Biochem/physiol Actions
该抗体会与大鼠雄激素受体发生交叉反应。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
N Yadav et al.
Minerva urologica e nefrologica = The Italian journal of urology and nephrology, 64(1), 35-49 (2012-03-10)
The embryonic development, growth and maturation of the prostate relies on androgens, the male sex steroids, acting through their cognate receptor, the androgen receptor (AR). This dependence on androgens continues in adult life, where AR signaling remains necessary for the
Urmila Santanam et al.
Genes & development, 30(4), 399-407 (2016-02-18)
Understanding new therapeutic paradigms for both castrate-sensitive and more aggressive castrate-resistant prostate cancer is essential to improve clinical outcomes. As a critically important cellular process, autophagy promotes stress tolerance by recycling intracellular components to sustain metabolism important for tumor survival.
Qing Xie et al.
The Journal of biological chemistry, 292(33), 13521-13530 (2017-07-07)
NK3 homeobox 1 (Nkx3.1), a transcription factor expressed in the prostate epithelium, is crucial for maintaining prostate cell fate and suppressing tumor initiation. Nkx3.1 is ubiquitously expressed in luminal cells of hormonally intact prostate but, upon androgen deprivation, exclusively labels
Jou-Ching Lin et al.
Poultry science, 103(6), 103669-103669 (2024-04-12)
In the poultry industry, broiler and layer strains are genetically selected for different purposes (e.g., high meat-yield and high egg-production). Genetic selection for productivity can have unintended consequences on the behavioral repertoire of the birds, including aggression. Alongside the increasing
X Q Sun et al.
Journal of neuroendocrinology, 25(6), 547-559 (2013-02-19)
The seminal vesicles are male accessory sex glands that contribute much of the seminal fluid volume. Previous studies have suggested that the majority of autonomic innervations to the rat seminal vesicles originate from the bilateral major pelvic ganglia. Many preganglionic
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