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安全信息

A9771

Sigma-Aldrich

白蛋白-荧光素异硫氰酸酯结合物

protein bovine

别名:

FITC-白蛋白

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About This Item

MDL编号:
MFCD00282182
UNSPSC代码:
12352204
eCl@ss:
42010201
NACRES:
NA.32

生物来源

protein from bovine

质量水平

200

表单

powder

标记范围

≥7 mol FITCmol albumin

技术

ELISA: suitable

溶解性

10 mM Tris pH 7.0: 10 mg/mL

应用

detection

储存温度

2-8°C

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一般描述

白蛋白占血浆蛋白的60％， 其由肝脏产生。它可调节胶体渗透压。白蛋白与脂肪酸、甲状腺激素和药物的运输、分布和代谢有关。它可以减少体重增加、阻塞肺水肿、减少腹水并降低组织水肿。

应用

白蛋白−异硫氰酸荧光素共轭物已被用于：
  • 作为对照，比较有或没有载体的蛋白质的细胞内递送效率
  • 蛋白质和细胞的荧光可视化
  • 作为ELISA中的封闭剂
  • 作为脑灌注和固定的化学剂
  • 体内 毛细血管-上皮渗透性实验
  • 共聚焦显微镜检查大鼠右心室（RV）脉管系统

制备说明

由结晶和冻干的白蛋白（A 4378）制备得到。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000406051
0000406053
0000406055
0000367743
0000367744

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the excitation and emission maxima for Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    The excitation maximum is 495 nm and the emission maximum is 520 nm.

  4. How is the FITC bound to the albumin in Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    The FITC is bound through the epsilon amino group of the lysine amino acid residues. Each lot number will have a varying number of FITC molecules bound.

  5. Is there any free FITC present in Product A9771, Albumin-fluorescein isothiocyanate conjugate?

    We would not expect there to be free FITC. The final product is dialyzed against distilled water using dialysis tubing with a molecular weight cutoff of 12,000 prior to it being lyophilized.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Antonio Brante et al.
The Journal of experimental biology, 212(Pt 14), 2287-2295 (2009-06-30)
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Tian L, et al
Journal of Molecular Medicine, 95(4), 381-393 (2017)
Molecular mechanisms of proteinuria
Molecular and Genetic Basis of Renal Disease (2008)
Highly Efficient Intracellular Protein Delivery by Cationic Polyethyleneimine-Modified Gelatin Nanoparticles
Chou MJ, et al.
Materials, 11(2), 301-301 (2018)
Airway epithelial KIF3A regulates Th2 responses to aeroallergens
Giridhar PV, et al.
Journal of Immunology, 23(7), 1600926-1600926 (2016)
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