膜联蛋白V 来源于人类胎盘

Research area: Apoptosis

Annexin V is a calcium-dependent phospholipid-binding protein. It is expressed in high concentration in cells with barrier functions, such as vascular endothelium, epithelial cells in bile ducts, placental trophoblasts, mammary ducts, renal tubules, and nasal epithelium.

Annexin V from human placenta has been used:

• to verify the involvement of phosphatidylserine in adhesion using flow cytometry
• to test adhesion specificity of phosphatidylserine
• for synthesis of NIR700-Annexin-V imaging probe
• in dynamic light scattering (DLS) assay to test the effects of various treatments on virion aggregation

膜联蛋白是普遍存在的同源蛋白，在钙存在的情况下结合磷脂。参与凋亡过程的细胞变化包括早期磷脂不对称的损失。这种现象是普遍存在的，不局限于刺激或哺乳动物细胞，也发生在昆虫和植物细胞中。在活细胞中，磷脂酰丝氨酸通过Mg²⁺ATP依赖酶氨磷脂转位酶传输至脂二层内部。发生细胞凋亡时，磷脂酰丝氨酸转运到细胞膜的外表面。磷脂酰丝氨酸从内膜表面到外膜表面的移动是细胞凋亡的早期指标，膜联蛋白V及其偶联物与磷脂酰丝氨酸相互作用且具有较强的特异性，可用于检测细胞凋亡。膜联蛋白V偶联物可用于检测明显早于基于DNA分析的凋亡细胞。 荧光染料标记的膜联蛋白V可应用于流式细胞术、荧光显微镜和激光扫描细胞术中。

It is a widely used marker for identifying apoptotic cells due to its high affinity for phosphatidylserine, which is normally located on the inner leaflets of membranes but moves to the cell surface during programmed cell death.

包装尺寸基于蛋白质含量

溶液溶于40 mM Tris-HCl，pH 7.5，含150 mM NaCl、1 mM DTT和0.05%叠氮化钠。