生物来源
bovine
质量水平
无菌性
aseptically filled
检测方案
28-32% protein basis (biuret)
形式
solution
纯化方式
cold ethanol fractionation
不包含
stabilizer or preservative
来源
USA origin
浓度
28-32% protein (biuret)
30% in saline
free fatty acid content
≤0.01%
技术
ELISA: suitable
electrophoresis: suitable
western blot: suitable
杂质
≤0.01% free fatty acid
UniProt登记号
异质活性
BT virus, none detected
VSV virus, none detected
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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一般描述
牛血清白蛋白(BSA)具有三个同源结构域,每个结构域将其细分为两个结构域(A和B)。它是一种α-螺旋球状蛋白,与人血清白蛋白具有相同的结构特征。
应用
牛血清白蛋白溶液已用于:
- 作为Dulbecco′s改良Eagle′s培养基的补充剂用于培养HepG2人肝癌症细胞
- 作为肝细胞复苏培养基的组分
- 作为绵羊体外复溶胚胎的融合和活化缓冲液的组分
生化/生理作用
牛血清白蛋白(BSA)是血液中主要的蛋白,具有钙结合位点并调节金属稳态。它是细胞培养基的一种重要组成部分,可促进胚胎干细胞(hESC)的分化。由于其具有高溶解度、低成本和纯度等优点,被广泛应用于化妆品和医药工业。 BSA在酶联免疫吸附测定(ELISA)中可用作封闭剂以防止抗原和抗体与微量滴定板的非特异性结合。
制备说明
采用冷乙醇沉淀分离法进行初次分级分离
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
含少量动物源组分生物产品
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