form
lyophilized powder
extent of labeling
10-14 μmol per mL
matrix
Sepharose 4B
matrix activation
cyanogen bromide
matrix attachment
amino group
matrix spacer
8 atoms (when ligands are coupled to the free carboxy groups. No coupling agent is necessary.)
swelling
1 g swells to 2.4-4.3 mL
storage temp.
2-8°C
Application
Activated CH-Sepharose 4B is a medium which binds to ligands containing amino groups via an 8 atom arm. It has been used with gel permeation chromatography to determine fractions of β-Glucans in different species of plant extract, which may display anti-diabetic activities.
Physical form
Lyophilized powder stabilized with lactose and dextran
Legal Information
Sepharose is a trademark of Cytiva
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
G D McIntyre et al.
The Biochemical journal, 211(2), 519-522 (1983-05-01)
A new affinity column for renin was prepared by coupling the isosteric peptide inhibitor of renin, H.77 (D-His-Pro-Phe-His-LeuR-Leu-Val-Tyr, where R is a reduced isosteric bond, -CH2-NH-), to activated 6-aminohexanoic acid-Sepharose 4B. Chromatography of a crude extract of human kidney cortex
R P Gupta et al.
Brain research, 878(1-2), 32-47 (2000-09-21)
Diisopropyl phosphorofluoridate (DFP) is an organophosphorus ester, which produces organophosphorus ester-induced delayed neuropathy (OPIDN) in hen and other sensitive species. A single dose of DFP (1.7 mg/kg, sc.) produces mild ataxia in 7-14 days in hens, which develops into severe
A C C F F De Paula et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 38(6), 885-893 (2005-06-04)
Beta-glucans are soluble fibers with physiological functions, such as interference with absorption of sugars and reduction of serum lipid levels. The objective of the present study was to analyze the distribution of beta-glucans in different tissues of the African grass
S J Cartwright et al.
The Biochemical journal, 221(2), 505-512 (1984-07-15)
Several beta-lactamases, enzymes that play an important part in antibiotic resistance, have been purified by affinity chromatography on boronic acid gels. The procedure is rapid, appears to be selective for beta-lactamases, and allows a one-step purification of large amounts of
I Palacios et al.
The EMBO journal, 16(22), 6783-6792 (1998-01-10)
Macromolecules that are imported into the nucleus can be divided into classes according to their nuclear import signals. The best characterized class consists of proteins which carry a basic nuclear localization signal (NLS), whose transport requires the importin alpha/beta heterodimer.
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