A8811
醛缩酶 来源于兔肌肉
ammonium sulfate suspension, 10-20 units/mg protein
别名:
D-果糖-1,6-二磷酸-D-甘油醛-3-磷酸裂解酶, 二磷酸果糖酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
10-20 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
form
ammonium sulfate suspension
specific activity
10-20 units/mg protein
foreign activity
glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%
storage temp.
2-8°C
Quality Level
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General description
兔醛缩酶存在三种亚型，包括来自肌肉的A型、肝脏B型和大脑相关C型。醛缩酶分子量为158 kDa，以四聚体形成存在。
Application
兔肌肉醛缩酶已用于：
- 在标准兔肌肉来源1-磷酸果糖激酶 (RPFK-1)实验中使用
- 作为青光眼组织来源代谢酶测定的标准品
- 用于人细胞系的果糖2,6-二磷酸分析
醛缩酶可用于将果糖1,6-二磷酸转化为磷酸二羟丙酮和3-磷酸甘油醛。来自兔肌肉中的醛缩酶已被用于DHAP C3的立体特异性去质子化。
Biochem/physiol Actions
醛缩酶与Wiskott-Aldrich综合征蛋白(WASP)相互作用，可调节肌动蛋白动态。它可逆转抗坏血酸对肌肉型乳酸脱氢酶(LDH-m4)的抑制作用。
醛缩酶可参与到糖原异生、卡尔文循环和糖酵解。来自兔肌肉中的醛缩酶属于I类醛缩酶，可形成共价席夫碱中间体。醛缩酶的活性位点位于α/β 8 筒形褶皱 的中央。
Physical form
2.5M (NH4)2SO4、0.01M Tris 溶液中的结晶悬浮液，pH 7.5，含 0.001M EDTA
Analysis Note
由缩二脲测定的蛋白质。
Other Notes
在pH 7.4、25 °C条件下，一个单位可在每分钟内将1.0 μ摩尔的的果糖1,6-二磷酸转化为磷酸二羟丙酮和3-磷酸甘油醛。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
Effect of lithium salts on lactate dehydrogenase, adenylate kinase, and 1-phosphofructokinase activities
Russell P, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 25(4), 551-556 (2010)
Structure of rabbit muscle aldolase at low resolution.
Sygusch J, et al.
The Journal of Biological Chemistry, 260(28), 15286-15290 (1985)
A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein
St-Jean M, et al.
The Journal of biological chemistry, 282(19), 14309-14315 (2007)
Clotilde LowKam et al.
The Journal of biological chemistry, 285(27), 21143-21152 (2010-04-30)
Tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes is a class I aldolase that exhibits a remarkable lack of chiral discrimination with respect to the configuration of hydroxyl groups at both C3 and C4 positions. The enzyme catalyzes the reversible cleavage of four
Geoffrey R Nosrati et al.
Biochemistry, 51(37), 7321-7329 (2012-08-23)
Catalytic atom maps (CAMs) are minimal models of enzyme active sites. The structures in the Protein Data Bank (PDB) were examined to determine if proteins with CAM-like geometries in their active sites all share the same catalytic function. We combined
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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