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主要文件

安全信息

A8549

Sigma-Aldrich

白蛋白,生物素标记 牛

lyophilized powder

别名:

Biotin labeled albumin

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352202
eCl@ss:
34058011
NACRES:
NA.25

生物来源

bovine

质量水平

方案

≥80% protein basis (biuret)

表单

lyophilized powder

标记范围

8-16 mol biotin per mol albumin

技术

bioconjugation: suitable

UniProt登记号

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

应用

生物素标记的牛白蛋白已被用作肌球蛋白V合成能力实验的组分。它也已用于提供用于中性亲和素包被的生物素标签,并在制备张力计系链(TGT)表面期间抑制非特异性细胞粘附。
生物素缀合的牛血清白蛋白选择性结合淋巴组织中的不同血管亚群,如荧光和酶组织化学技术所证实的,因此提供了用于鉴定淋巴组织中选定的微血管内皮的组织化学探针。在脾脏中,生物素-BSA与红色牙髓窦结合,但不与白色牙髓血管(包括中央小动脉)或边缘窦结合。在胸腺中,显著的结合仅限于皮质髓质区域的毛细血管。

外形

含有柠檬酸钠缓冲盐

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

含少量动物源组分生物产品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Product A8549, Albumin, biotin labeled bovine, soluble in?

    This product is soluble in water. This product contains sodium citrate buffer salts, so addition of a buffer is probably not necessary; just adding water is sufficient.

  4. What is the solution stability of Product A8549, Albumin, biotin labeled bovine?

    Sigma Aldrich has not tested the stability of this product in solution.  It is recommended to prepare fresh solutions.

  5. What is the linker between the BSA and the biotin for Product A8549, Albumin, biotin labeled bovine?

    There is no linker or "spacer". The link between the BSA and the biotin is an amide bond connecting an amino group on the BSA to the carboxy group on the biotin.

  6. What is the method of preparation for Product A8549, Albumin, biotin labeled bovine?

    The method of preparation of this product is considered proprietary by Sigma-Aldrich.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Do you have the amino acid sequence for bovine serum albumin?

    Bovine Serum Albumin is produced as a protein of 607 amino acids. The signal peptide (amino acids 1-18) and the propeptide (amino acids 19-22) are cleaved to make the mature form.  The mature form of BSA is 583 amino acids (amino acids 25-607, Uniprot P02769).  The sequence for the mature protein is below:DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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