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Merck
CN

A8354

Anti-Beta (β)-Amyloid antibody

mouse monoclonal, NAB 228

别名:

抗 Aβ

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00803867

主要文件

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产品名称

抗-β淀粉样蛋白抗体，小鼠单克隆 小鼠抗, clone NAB 228, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

NAB 228, monoclonal

form

buffered aqueous solution

mol wt

antigen ~110 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using cell extract of the human embryonal carcinoma NTERA-2 (NT2/D1) cells, treated for 2-3 weeks with 10 μM retinoic acid

isotype

IgG2a

UniProt accession no.

P05067

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... APP(351)

相关类别

Application

单克隆抗 β-淀粉样蛋白抗体已用于 IgG1 和 IgG2a 同种型抗体的滴定。它也被用于合成抗体功能化的磁性纳米颗粒。
小鼠单克隆抗-β-淀粉样蛋白抗体已可用于蛋白质印迹分析。本品还可用于免疫组织化学、免疫沉淀、间接ELISA和微阵列研究。
此抗体已成功使用的应用，以及相关的同行评审论文如下。
蛋白免疫分析（Western Blotting）（1篇文章）

Biochem/physiol Actions

β-淀粉样蛋白基因是胰岛素降解酶（IDE）的底物。它在阿尔茨海默病（AD）和2型糖尿病（DM2）的发病机制中发挥主要作用。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

β-淀粉样蛋白前体蛋白 (APP) 是由蛋白水解酶β-分泌酶 (BACE1) 和 γ-分泌酶依次裂解，生成以Aβ1-42和 Aβ1-40型为主的β-淀粉样蛋白 (Aβ) 肽。分泌的Aβ肽通过再吸收机制和之后的内体降解过程降解，或通过胞外胰岛素降解酶降解。Aβ的胞外积累导致聚集体和原纤维的形成，最终形成淀粉样蛋白沉着物神经炎斑，其是阿尔茨海默病 (AD) 的标志物。
淀粉样β-肽（Aβ), 一种 含38至 43 个氨基酸的肽，来源于β-淀粉样前体蛋白（APP）。β-淀粉样蛋白基因位于人染色体21q21上。APP是一种I型跨膜蛋白。它在内质网（ER）中产生，然后在高尔基体的协助下迁移至反面高尔基网（trans-Golgi-network，TGN）。
淀粉样蛋白是由错误折叠的蛋白质和多肽组成的不溶性蛋白聚集体。淀粉样蛋白沉积与多种神经退行性疾病有关。
该抗体可识别人 β-淀粉样肽、全长淀粉样前体蛋白 (APP)、可溶性 APP（sAPPβ′和 sAPPα）、C99 裂解 形式和 Aβ(1-40/42)，但不是可溶性 APP 形式 sAPPβ。

Immunogen

相当于人 β1-11 氨基酸的合成肽——淀粉样蛋白。

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000426954
0000426954
0000426953
0000426953
0000420000

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Do you have a picture of the western blot including the amount of protein loaded and antibody dilution used for Product A8354 - Monoclonal Anti-β-Amyloid antibody produced in mouse?

    Attaced is a QC work sheet that includes al the information your customer required. the extract used is NT2 (human embryonal Carcinoma) cells treated with 10 micromolar Retinoic acid for 2-3 weeks

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Jose Gregorio Salazar et al.
Antioxidants (Basel, Switzerland), 10(3) (2021-03-07)
Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer's disease (AD). Paraoxonase-1 and -3 (PON1 and PON3) protein expression was demonstrated in tissue with no PON1 or PON3 gene expression. In the present study, we
Thai Thao Ly et al.
Biosensors, 11(10) (2021-10-23)
An extraordinary optical transmission fibre-optic surface plasmon resonance biosensing platform was engineered to improve its portability and sensitivity, and was applied to monitor the concentrations of monoclonal antibodies (Mabs). By refining the fabricating procedure and changing the material of the
Joana Poejo et al.
International journal of molecular sciences, 22(4) (2021-03-07)
Lipid rafts are a primary target in studies of amyloid β (Aβ) cytotoxicity in neurons. Exogenous Aβ peptides bind to lipid rafts, which in turn play a key role in Aβ uptake, leading to the formation of neurotoxic intracellular Aβ
Shen-Qing Zhang et al.
Frontiers in molecular neuroscience, 13, 613421-613421 (2021-02-02)
Clinical studies have found that some Alzheimer's disease (AD) patients suffer from Cushing's syndrome (CS). CS is caused by the long-term release of excess glucocorticoids (GCs) from the adrenal gland, which in turn, impair brain function and induce dementia. Thus
An overview of APP processing enzymes and products
Chow V W, et al.
Neuromolecular Medicine, 12(1), 1-12 (2010)
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