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安全信息

A7888

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, suitable for RIA, pH 5.2, ≥96%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS号:
9048-46-8
EC 号:
232-936-2
MDL编号:
MFCD00130384
UNSPSC代码:
12352202
NACRES:
NA.27

生物来源

bovine

质量水平

200

等级

RIA grade

检测方案

≥96%

形式

lyophilized powder

质量

suitable for RIA

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

poly bottle of

来源

USA origin

技术

blood typing: suitable

缺失

≤5%

pH值（酸碱度）

5.2

溶解性

water: soluble (40 mg/ml)

适用性

suitable for insulin RIA procedures

UniProt登记号

P02769

异质活性

BT virus, none detected
VSV virus, none detected

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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相关类别

一般描述

牛血清白蛋白（BSA）是一种由每个均带有两个亚结构域的三个结构域所组成的66 kDa蛋白。它是一种α-螺旋、球状且非糖基化的蛋白。

应用

牛血清白蛋白已用于：
  • 酶联免疫吸附试验（ELISA）的封闭剂（154）
  • 作为窦前卵泡培养的Dulbecco PBS培养基的组分（155）
  • 作为卵母细胞授精的改良Tris缓冲培养基（mTBM）的组分（156）

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
牛血清白蛋白（BSA）是一种药物、激素和脂肪酸的转运蛋白。它可在酶联免疫吸附测定（ELISA）中可用作封闭剂以防止抗原和抗体与微量滴定板的非特异性结合。BSA的半胱氨酸和酪氨酸残基对金属结合具有很高的亲和力。

特点和优势

  • 热休克分级
  • 适用于放射免疫分析（RIA）

注意

在某些程序中，可能会产生高达0.1 μ单位/毫克的胰岛素空白。

制备说明

使用热休克分级制备
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product A7888, Albumin from bovine serum?

    BSA consists of 583 amino acid residues.  The molecular weight calculated by NIST is 66429.03. It has also been calculated by GPMAW (General Protein Mass Analysis for Windows) as 66429.94.

  4. What does RIA grade mean in the description of Product A7888, Albumin from bovine serum?

    RIA grade means that this product has been shown suitable for radioimmunoassays.

  5. At what concentration is Product A7888, Albumin from bovine serum (BSA), soluble in water?

    We have not established an upper limit of solubility for this product in water.  At 40 mg/mL, an aqueous solution of  this product may light yellow to green and slightly hazy.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

K B Cullberg et al.
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Xiao Y and Isaacs SN
Journal of Immunological Methods, 384(1-2), 148-151 (2012)
Sampada Chande et al.
Scientific reports, 10(1), 3069-3069 (2020-02-23)
Low blood phosphate (Pi) reduces muscle function in hypophosphatemic disorders. Which Pi transporters are required and whether hormonal changes due to hypophosphatemia contribute to muscle function is unknown. To address these questions we generated a series of conditional knockout mice
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Bovine serum albumin interactions with metal complexes
Topalua T, et al.
Clujul Medical (1957), 87(4), 215-215 (2014)
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