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Merck
CN

A7646

Sigma-Aldrich

三磷酸腺苷双磷酸酶 来源于马铃薯

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

别名:

腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

potato

质量水平

类型

Grade III

形式

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

分子量

~49 kDa by gel filtration

组成

Protein, 30-60%

溶解性

H2O: soluble 10 mg/mL, slightly hazy

应用

diagnostic assay manufacturing

异质活性

Acid Phosphatase ≤5% of base activity

储存温度

−20°C

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应用

三磷酸核苷和二磷酸核苷的水解酶。对于有机二磷酸和三磷酸的水解,最适 pH 为 6,对于无机底物,最适 pH 为 5.1 该酶已被用于评估 ATP 参与肠道固有层 Th17 细胞分化的可能性。
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶:一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应:ATP → ADP+Pi → AMP+2Pi

生化/生理作用

在所有的真核生物和一些原核生物中都存在着这种酶。来自马铃薯的三磷酸腺苷双磷酸酶,对生长发育具有至关重要的调节作用。在神经刺激后,作为神经递质参与突触 ATP 的失活,并抑制 ADP 诱导的血小板聚集以防止血栓形成。活性需要二价金属离子,钙离子在 5 mm 时活性最好,发现蛋白分子量约为 45 kDa。

包装

按 atp 酶单位销售

其他说明

高比率和低比率同工酶混合物。

质量

含量≤5% 酸性磷酸酶。

单位定义

在pH 6.5、30℃条件下,一单位酶每分钟可从ATP或ADP释放1.0 μmM无机磷酸盐。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

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H J Kruse et al.
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