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生物来源
Porcine cerebral cortex
质量水平
描述
Sodium and Potassium-Activated
形式
lyophilized powder
比活
≥0.3 units/mg protein, pH 7.8
组成
Protein, 5.0-25.0% biuret
技术
inhibition assay: suitable
储存温度
−20°C
基因信息
pig ... ATP1A1(397481) , ATP1B1(396898)
应用
ATP酶用于从ATP中释放无机磷。来自猪大脑皮质的ATP酶已用于Na / K-ATP酶活性的抑制测定。
生化/生理作用
这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。蟾酥毒基和其他强心类固醇可能抑制 Na +/K +-atp 酶活性，诱导 HL60、U937 和 ML1 细胞分化。
这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。 它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。
单位定义
如果存在Na+, K+和Mg2+，在 pH值为 7.8、37 C 条件下，每分钟从 ATP 释放 1.0 μmol 无机磷
外形
含有90%蔗糖、0.4%EDTA Na 4+和0.06%NaCl的冻干粉末
制备说明
碘化钠提取部分，Nakao 等人， Biochem. Biophys. Res. Commun., 19, 755 (1965).
抑制剂
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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