A7510
腺苷 5′-三磷酸酶 来源于猪大脑皮质
lyophilized powder, ≥0.3 units/mg protein, pH 7.8
别名:
ATP 磷酸水解酶, ATP酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥0.3 units/mg protein, pH 7.8
Biological source:
Porcine cerebral cortex
biological source
Porcine cerebral cortex
description
Sodium and Potassium-Activated
form
lyophilized powder
specific activity
≥0.3 units/mg protein, pH 7.8
composition
Protein, 5.0-25.0% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
Quality Level
Gene Information
pig ... ATP1A1(397481), ATP1B1(396898)
Application
ATP酶用于从ATP中释放无机磷。来自猪大脑皮质的ATP酶已用于Na / K-ATP酶活性的抑制测定。
Biochem/physiol Actions
这种膜酶将 ATP 水解为 ADP 和正磷酸盐。水解过程伴随着钠离子和钾离子交换穿过质膜。这种酶几乎存在于高等生物的所有组织中，但在肾脏中含量最多，因为它从肾小球滤液中重吸收钠离子。Na + ,K +-atp 酶受细胞内钙离子变化的影响。它也对哇巴因敏感，并被钒酸盐和（体外）SPAI-1（一种从猪十二指肠分离的肽）抑制。研究表明，它可通过蛋白激酶 A (PKA) 的磷酸化进行调节。蟾酥毒基和其他强心类固醇可能抑制 Na +/K +-atp 酶活性，诱导 HL60、U937 和 ML1 细胞分化。
Physical form
含有90%蔗糖、0.4%EDTA Na 4+和0.06%NaCl的冻干粉末
Preparation Note
碘化钠提取部分，Nakao 等人， Biochem. Biophys. Res. Commun., 19, 755 (1965).
Other Notes
如果存在Na+, K+和Mg2+，在 pH值为 7.8、37 C 条件下，每分钟从 ATP 释放 1.0 μmol 无机磷
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
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