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安全信息

A7420

Sigma-Aldrich

淀粉葡萄糖苷酶 来源于黑曲霉

greener alternative

lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

别名:

1,4-α-D-葡聚糖葡萄糖水解酶, 糖化淀粉酶, 葡萄糖淀粉酶

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About This Item

CAS号:
9032-08-0
EC 号:
3.2.1.3 (BRENDA, IUBMB)
EC 号:
232-877-2
MDL编号:
MFCD00081350
UNSPSC代码:
12352204
NACRES:
NA.54

形式

lyophilized powder

质量水平

200

比活

30-60 units/mg protein (biuret)

组成

protein, ≥80%

环保替代产品特性

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

杂质

≤0.02% glucose

环保替代产品分类

, Enabling

储存温度

−20°C

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相关类别

一般描述

我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对淀粉乙醇研究进行了优化，可提高能效并防止浪费。有关详细信息，请参阅 《Biofiles》的文章。

应用

来自黑曲霉的淀粉葡萄糖苷酶用于水解α-D-葡糖苷。 它可用于啤酒的酿造以及面包和果汁的生产。 Sigma的淀粉葡萄糖苷酶被用于将糖原水解成葡萄糖单体，以研究骨骼肌中的脂质积累。

生化/生理作用

来自黑曲霉的淀粉葡萄糖苷酶能够水解低聚糖的α-D-(1-4)、α-D-(1-6)和α-D-(1-3)糖苷键。淀粉葡萄糖苷酶是一种胞外酶，可将淀粉转化成糊精和葡萄糖。该酶在淀粉加工工业中用于由玉米糖浆商业生产D-葡萄糖。

单位定义

在pH 4.5、55℃条件下，一单位酶在3 min内可从淀粉中释放出1.0 mg葡萄糖。

外形

含有低于0.02％葡萄糖的冻干粉

其他说明

有关复合碳水化合物分的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

抑制剂

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of Product A7420,  Amyloglucosidase from Aspergillus niger?

    Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).

  6. Does Product A7420,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?

    The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.

  7. How can a solution of Product A7420,  Amyloglucosidase from Aspergillus niger, be prepared?

    A 1 mg/mL solution may be prepared in water.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Youngmi Kim et al.
Bioresource technology, 99(12), 5165-5176 (2007-11-09)
DDGS and wet distillers' grains are the major co-products of the dry grind ethanol facilities. As they are mainly used as animal feed, a typical compositional analysis of the DDGS and wet distillers' grains mainly focuses on defining the feedstock's
Yi-Chen Lin et al.
PloS one, 12(5), e0177115-e0177115 (2017-05-05)
It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific
Erick Perera et al.
Scientific reports, 10(1), 18676-18676 (2020-10-31)
The aim of this work was to evaluate two functional feeds for the gilthead seabream, Sparus aurata, containing low inclusion of two microalgae-based products (LB-GREENboost, LBGb; and LB-GUThealth, LBGh). Fish (12-13 g) were fed for 13 weeks a control diet or one
Alisa K Manning et al.
Frontiers in genetics, 11, 615-615 (2020-08-06)
Causal transcripts at genomic loci associated with type 2 diabetes (T2D) are mostly unknown. The chr8p23.1 variant rs4841132, associated with an insulin-resistant diabetes risk phenotype, lies in the second exon of a long non-coding RNA (lncRNA) gene, LOC157273, located 175
M Ramadas et al.
World journal of microbiology & biotechnology, 12(3), 267-271 (1996-05-01)
Amyloglucosidase (AMG) was produced by Aspergillus niger in solid-state fermentation (SSF), submerged fermentation (SmF) and an aqueous, two-phase system of polyethyleneglycol (PEG) and salt. In SSF, a fed-batch mode of operation gave a yield of 64 U/ml compared with 44
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实验方案

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

淀粉葡萄糖苷酶的酶学测定（EC 3.2.1.3）

本操作程序可用于使用淀粉作为底物测定淀粉葡萄糖苷酶的活性。

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