一般描述
抗-V5 琼脂糖亲和凝胶由与溴化氰活化的珠状琼脂糖偶联的单克隆抗-V5 抗体组成。 该抗体识别转染的哺乳动物细胞中表达的 V5 标记的重组融合蛋白。
抗-V5琼脂糖亲和凝胶由纯化的单克隆抗-V5抗体免疫球蛋白部分与溴化氰活化的珠状琼脂糖偶联组成，单克隆抗-V5抗体从 V5-10杂交瘤腹水中分离。纯化的单克隆抗体固定在琼脂糖上。
特异性
识别 V5 标签（GKPIPNPLLGLDST）融合蛋白
应用
鼠源抗V5琼脂糖亲和凝胶抗体用于免疫沉淀和重组蛋白纯化。
生化/生理作用
重组 DNA 技术可将目的基因附着在特定序列或基因上，提供′亲和柄′设计，选择性识别和纯化目的蛋白。 在给定基因中添加V5标签序列（GKPIPNPLLGLDST）会产生稳定的融合产物，这一产物似乎不会干扰蛋白的生物活性或干扰标记产物的生物分布。研究人员已经设计出许多重组蛋白表达衍生自猴副流感病毒V5的短序列，这些短序列称为V5-标签。抗“标签”琼脂糖结合物可以促进检测、分离和纯化这些蛋白质。
外形
将珠状琼脂糖（1：1v / v）悬浮于含有 15mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水中。
储存及稳定性
为保证连续使用和延长存储时间，请在2°C至8°C条件下存储。请勿冷冻。
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闪点(°F)
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闪点(°C)
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