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Merck
CN

A7058

Sigma-Aldrich

抗多组氨酸−小鼠单克隆甲状腺过氧化物酶抗体 小鼠抗

clone HIS-1, purified from hybridoma cell culture

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别名:
聚组氨酸单克隆抗体, 6 His表位标签单克隆抗体, 6xHis标签单克隆抗体, HHHHHH表位标签单克隆抗体, Hexa His标签单克隆抗体, His6标签单克隆抗体, 组氨酸标签单克隆抗体, 聚组氨酸标签单克隆抗体
UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

mouse

质量水平

偶联物

peroxidase conjugate

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

HIS-1, monoclonal

形式

lyophilized powder

包装

vial of 0.5 mL

浓度

5-11 mg/mL

技术

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

同位素/亚型

IgG2a

储存温度

2-8°C

靶向翻译后修饰

unmodified

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一般描述

抗多组氨酸过氧化物酶单克隆缀合抗体可识别天然和变性形式的合成多组氨酸或多组氨酸标记的融合蛋白。 该产品可与原核生物pET、pRSET和pTrc表达载体表达的融合蛋白反应。

特异性

该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。

免疫原

重组聚组氨酸标签融合蛋白。

应用

抗体适用于免疫印迹。 工作稀释度1:2000
也适用于斑点印迹分析和ELISA

外形

从含有 1% 牛血清白蛋白和 0.05% MIT的 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 冻干。

制备说明

通过Avrameas, S.等人在Scand. J. Immunol., 8, Suppl. 7, 7 (1978)中描述的两步戊二醛法制备。

重悬

抗体缀合物应用0.5 ml去离子水复溶。

法律信息

该产品受专利DE 19507166和国外等效文件的保护,独家授权给德国QIAGEN GmbH、Qiagen Strasse 1、D-40724 Hilden。

危险分类

Skin Sens. 1

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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