biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
storage temp.
2-8°C
target post-translational modification
unmodified
General description
抗多组氨酸过氧化物酶单克隆缀合抗体可识别天然和变性形式的合成多组氨酸或多组氨酸标记的融合蛋白。 该产品可与原核生物pET、pRSET和pTrc表达载体表达的融合蛋白反应。
Immunogen
重组聚组氨酸标签融合蛋白。
Application
抗体适用于免疫印迹。 工作稀释度1:20
也适用于斑点印迹分析和ELISA
也适用于斑点印迹分析和ELISA
Biochem/physiol Actions
该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
Physical form
从含有 1% 牛血清白蛋白和 0.05% MIT的 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 冻干。
Preparation Note
抗体缀合物应用0.5 ml去离子水复溶。
通过Avrameas, S.等人在Scand. J. Immunol., 8, Suppl. 7, 7 (1978)中描述的两步戊二醛法制备。
Other Notes
Legal Information
该产品受专利DE 19507166和国外等效文件的保护,独家授权给德国QIAGEN GmbH、Qiagen Strasse 1、D-40724 Hilden。
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A7058-1VL | 04061838443632 |
