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生物来源
mouse
质量水平
偶联物
peroxidase conjugate
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
HIS-1, monoclonal
表单
lyophilized powder
包装
vial of 0.5 mL
浓度
5-11 mg/mL
技术
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
同位素/亚型
IgG2a
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
抗多组氨酸过氧化物酶单克隆缀合抗体可识别天然和变性形式的合成多组氨酸或多组氨酸标记的融合蛋白。 该产品可与原核生物pET、pRSET和pTrc表达载体表达的融合蛋白反应。
特异性
该抗体可识别合成聚组氨酸，以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
免疫原
重组聚组氨酸标签融合蛋白。
应用
抗体适用于免疫印迹。 工作稀释度1:20
也适用于斑点印迹分析和ELISA
也适用于斑点印迹分析和ELISA
外形
从含有 1% 牛血清白蛋白和 0.05% MIT的 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 冻干。
制备说明
通过Avrameas, S.等人在Scand. J. Immunol., 8, Suppl. 7, 7 (1978)中描述的两步戊二醛法制备。
重悬
抗体缀合物应用0.5 ml去离子水复溶。
其他说明
法律信息
该产品受专利DE 19507166和国外等效文件的保护，独家授权给德国QIAGEN GmbH、Qiagen Strasse 1、D-40724 Hilden。
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警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Yang Zhang et al.
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Directed Evolution of a Highly Specific FN3 Monobody to the SH3 Domain of Human Lyn Tyrosine Kinase.
Renhua Huang et al.
PloS one, 11(1), e0145872-e0145872 (2016-01-06)
Affinity reagents of high affinity and specificity are very useful for studying the subcellular locations and quantities of individual proteins. To generate high-quality affinity reagents for human Lyn tyrosine kinase, a phage display library of fibronectin type III (FN3) monobodies
Vincenzo Lionetti et al.
BMC plant biology, 15, 6-6 (2015-01-20)
Fusarium graminearum, one of the causal agents of Fusarium Head Blight (FHB, scab), leads to severe losses in grain yield and quality due to the production of mycotoxins which are harmful to human and livestock. Different traits for FHB resistance
Laurence Guglielmi et al.
Methods in molecular biology (Clifton, N.J.), 562, 215-224 (2009-06-26)
The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv.
Philippe Thullier et al.
PloS one, 8(5), e65855-e65855 (2013-06-07)
The lethal toxin (LT) of Bacillus anthracis, composed of the protective antigen (PA) and the lethal factor (LF), plays an essential role in anthrax pathogenesis. PA also interacts with the edema factor (EF, 20% identity with LF) to form the
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