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文件

A7054

Sigma-Aldrich

ACCUSPIN™ 系统-Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

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MDL编号:
MFCD01092091
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

400

无菌性

aseptically filled

形式

solution

保质期

Expiry date on the label.

包装

pkg of package 12 tubes

密度

1.077 g/mL

应用

hematology
histology

储存温度

2-8°C

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相关类别

一般描述

ACCUSIN System-Histopaque-1077产品用于分离淋巴细胞和其他单核细胞。分离介质Histopaque-1077是一种无菌过滤的、经内毒素检测的聚蔗糖和泛影酸钠溶液，密度调整为1.077 g/mL。ACCUSPIN系统-Histopaque 1077采用γ辐照的50 mL聚丙烯离心管，专门设计多孔高密度聚乙烯屏障（“筛板”）隔开两个腔室。每管无菌灌装15 mL的Histopaque 1077。 每管可分离15-30 mL抗凝血。

应用

ACCUSIN System-Histopaque 1077使用密度梯度分离介质从全血和骨髓中分离淋巴细胞和其他单个核细胞。Histopaque-1077用于：

  • 人淋巴细胞抗原(HLA)分型
  • T、B和‘裸’淋巴细胞计数前的起始分离。
  • 制备用于细胞培养和细胞毒性试验的纯淋巴细胞悬液。
  • 分离和纯化外周血单个核细胞(PBMC)和骨髓单个核细胞(BMMC)
  • 分离人类淋巴细胞
  • 分离全血中乳癌循环肿瘤细胞(CTC)
ACCUSPIN System-HISTOPAQUE-1077 采用专门设计的离心管，两个腔室由多孔高密度聚乙烯屏障（“熔块”）隔开。下部腔室含有 HISTOPAQUE-1077，可添加抗凝全血而不存在与分离介质混合的风险。离心时，全血通过玻璃料下降，与玻璃料下方的 HISTOPAQUE-1077 接触，使血液成分明显分离。红细胞聚集，粒细胞变得略微高渗，增加其沉降率，导致 ACCUSPIN 管底部出现颗粒。淋巴细胞和其他单核细胞，即单核细胞，保留在血浆-HISTOPAQUE-1077 界面。

特点和优势

ACCUSPIN™ System-Histopaque®-1077具有很多优点，例如：
  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活性
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • Histopaque 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少 2 年

原理

下部分含有HISTOPAUE-1077，保证添加抗凝全血时不会与分离介质混合。离心后，全血下沉通过筛板，与筛板下方的HISTOPAQUE-1077接触，从而将血液成分完全分离。红细胞聚集，粒细胞变得略微高渗，增加其沉降率并导致ACCUSPIN管底部出现沉淀。淋巴细胞和单核白细胞等其他单核细胞留在血浆-HISTOPAQUE-1077分界面。

数量

包装内含 12 根预填充管

法律信息

Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the most commonly used anticoagulants for the Accuspin tubes (Product A7054)?

    EDTA or heparin are the most commonly used anticoagulants.  Heparin however should not be used when performing RNA assays.  EDTA can interfere with certain calcium binding assays.  Other anticoagulants have been reported to give acceptable results.

  4. Does peripheral blood need to be diluted prior to loading into the Accuspin tubes (Product A7054)?

    No.  The blood may either be used without dilution or with a 1:2 dilution.   In house studies at Sigma Aldrich have showed no decrease in recovery for peripheral blood specimens loaded directly into the Accuspin tubes.

  5. May buffy coats or donor packs with reduced red cell numbers be used with Accuspin tubes (Product A7054)?

    No.  For the mononuclear cells to band above the "frit", the specimen must contain a sufficient number of red cells to displace the density gradient above the "frit".

  6. Can granulocytes be collected using the Accuspin tubes (Product A7054)?

    No.  After centrifugation, the granulocytes will end up at the bottom of the Accuspin tube - just above the packed red blood cells.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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默克密度梯度介质_细胞分离-默克生命科学

我们提供完整的密度梯度介质产品线，用于分离或提取白细胞、病毒、DNA、RNA、细胞器以及许多其他应用。我们的产品线包括用于在研究实验室中分离白细胞的Histopaque®碘化梯度介质，用于哺乳动物细胞分离的多糖介质，用于哺乳动物细胞和细胞器分离的胶体二氧化硅介质，以及用于DNA、病毒和蛋白质分离的无机盐介质。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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