跳转至内容
Merck
CN

A7030

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

登录查看公司和协议定价


About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.27

生物来源

bovine

质量水平

检测方案

≥98%

形式

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

来源

USA origin

游离脂肪酸含量

≤0.02%

技术

ELISA: suitable
blood typing: suitable
ligand binding assay: suitable

杂质

essentially globulin free

缺失

≤5%

pH值(酸碱度)

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

protease ≤0.005 U/mg

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

正在寻找类似产品? 访问 产品对比指南

一般描述

牛血清白蛋白是一种在调节血液胶体渗透压中起关键作用的蛋白质。一种由583个氨基酸构成的水溶性蛋白,BSA的计算分子量为66,430 道尔顿。六个α-螺旋形成了BSA的三个同源结构域。BSA可依赖于pH进行可逆的构象异构化。蛋白的天然结构在加热时会变得具有反应性和灵活性。

该 BSA 产品针对以下特性经过专门评估和测试:
  • 非常低的蛋白酶含量/无蛋白酶
  • 非常低的脂肪酸含量/无脂肪酸

无蛋白酶 BSA 特别适用于以下应用:
  • 酶测定
  • 蛋白质测定
  • 蛋白酶敏感型技术,包括:
a. 酶免疫法(EIA)
b. 核酸杂交
c. 放射免疫测定(RIA)

应用

牛血清白蛋白已用于:
  • 作为人多能干细胞分化培养基的成分
  • 酶联免疫吸附试验(ELISA)和夹心 ELISA
  • 使胎儿皮层细胞具有通透性

这种 A7030 BSA 专门用作基于肽的 ELISA 测定中的封闭缓冲液成分,用于分析 COVID-19 / SARS-CoV-2 样品(Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020))。

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特点和优势

  • 热休克分级
  • 无蛋白酶
  • 无脂肪酸
  • 几乎不含球蛋白

制备说明

热激分级分离法制取
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

通常也和此产品一起购买

产品编号
说明
价格

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

  1. What is the molecular weight of A7030, BSA?

    Albumin has a molecular weight of 66,430 g/mol as a single polypeptide chain.

  2. Is Product No. A7030, BSA fraction V?

    All albumins are fraction V. Based on the fractionation of serum, fraction I is fibrinogen, fraction II is gamma-globulins, fraction III is beta-globulins, fraction IV is alpha-globulins, and fraction V is albumin.

  3. What does "fatty acid free" mean as it relates to BSA albumin, Product A7030?

    Albumin has 4 hydrophobic binding domains but the normal load is 1 to 2 molecules per molecule of albumin.  The normal level of fatty acids for albumin is 0.05 % +/- 0.03 %. Fatty acid free albumin will have less than 0.005 % fatty acids.

  4. How many lysine residues does A7030 albumin have?

    Bovine albumin will have 59 lysine residues but not all of them are accessible for conjugation.

  5. What is the half life of Product A7030, BSA albumin in the circulation?

    The normal half-life of circulating albumin is 19 days.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Fixed single-cell transcriptomic characterization of human radial glial diversity
Elliot R Thomsen
Nature Methods, 13(1), 87-93 (2016)
Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling.
Hohwieler M
Gut, 66(3), 473-486 (2017)
Xueying Zhao et al.
International journal of molecular sciences, 20(14) (2019-08-03)
Diabetic nephropathy is increasingly recognized as a major contributor to kidney failure in patients with obesity and type 2 diabetes. This study was designed to identify the molecular mediators of kidney injury associated with metabolic syndrome with or without hyperglycemia.
C H J Albers-Wolthers et al.
PloS one, 12(6), e0179156-e0179156 (2017-06-27)
Kisspeptins (KPs) and their receptor (GPR54 or KiSS1R) play a key-role in regulation of the hypothalamic-pituitary-gonadal axis and are therefore interesting targets for therapeutic interventions in the field of reproductive endocrinology. As dogs show a rapid and robust LH response
Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay
Jiuping Ji
PLoS ONE (2017)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门