生物来源
bovine
质量水平
检测方案
≥98%
形式
lyophilized powder
分子量
~66 kDa
纯化方式
heat shock fractionation
来源
USA origin
游离脂肪酸含量
≤0.02%
技术
ELISA: suitable
blood typing: suitable
ligand binding assay: suitable
杂质
essentially globulin free
缺失
≤5%
pH值(酸碱度)
7
溶解性
water: soluble (40 mg/ml)
UniProt登记号
异质活性
protease ≤0.005 U/mg
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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一般描述
该 BSA 产品针对以下特性经过专门评估和测试:
- 非常低的蛋白酶含量/无蛋白酶
- 非常低的脂肪酸含量/无脂肪酸
无蛋白酶 BSA 特别适用于以下应用:
- 酶测定
- 蛋白质测定
- 蛋白酶敏感型技术,包括:
b. 核酸杂交
c. 放射免疫测定(RIA)
应用
- 作为人多能干细胞分化培养基的成分
- 酶联免疫吸附试验(ELISA)和夹心 ELISA
- 使胎儿皮层细胞具有通透性
这种 A7030 BSA 专门用作基于肽的 ELISA 测定中的封闭缓冲液成分,用于分析 COVID-19 / SARS-CoV-2 样品(Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020))。
生化/生理作用
特点和优势
- 热休克分级
- 无蛋白酶
- 无脂肪酸
- 几乎不含球蛋白
制备说明
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
What is the molecular weight of A7030, BSA?
Albumin has a molecular weight of 66,430 g/mol as a single polypeptide chain.
Is Product No. A7030, BSA fraction V?
All albumins are fraction V. Based on the fractionation of serum, fraction I is fibrinogen, fraction II is gamma-globulins, fraction III is beta-globulins, fraction IV is alpha-globulins, and fraction V is albumin.
What does "fatty acid free" mean as it relates to BSA albumin, Product A7030?
Albumin has 4 hydrophobic binding domains but the normal load is 1 to 2 molecules per molecule of albumin. The normal level of fatty acids for albumin is 0.05 % +/- 0.03 %. Fatty acid free albumin will have less than 0.005 % fatty acids.
How many lysine residues does A7030 albumin have?
Bovine albumin will have 59 lysine residues but not all of them are accessible for conjugation.
What is the half life of Product A7030, BSA albumin in the circulation?
The normal half-life of circulating albumin is 19 days.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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