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Merck
CN

A6982

Sigma-Aldrich

HydroMatrix细胞培养多肽支架

mixture, powder

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About This Item

UNSPSC代码:
12352207

生物来源

synthetic

质量水平

表单

powder

储存温度

−20°C

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应用

HydroMatrix 已被证明可在许多细胞类型培养中促进细胞生长和迁移,包括神经干细胞、神经元、胶质细胞、星形胶质细胞、成纤维细胞和角质形成细胞。

组分

HydroMatrix 是一种肽纳米纤维三维支架,可促进细胞生长和迁移。当使用高温或高离子强度时,它利用从流体前体自组装成高度交联的肽水凝胶的特定肽。通过诱导从溶液到凝胶的快速转化,生成肽纳米纤维支架。调整 HydroMatrix 溶液的浓度,研究人员可以控制三维架构的灵活性,以满足其应用的特定需求。

注意

该冻干品置于 -20℃ 下,在该温度下可稳定多年。水凝胶应储存于 -70°C,使有效期最大化。1%(w/v)储备溶液应储存在 -20°C,并可稳定 3-6 个月。

制备说明

该产品以冻干粉形式提供。加入无菌水可以制备 10mg/mL 的 1%(w/v)储备溶液,得到刚性凝胶。0.5%(w/v)溶液将产生更柔软的凝胶,更适合细胞迁移和血管生成。0.15%-0.25% 范围内的较低浓度更适用于神经元等多种细胞类型。在水溶液中,HydroMatrix 的 pH 值为 2.5,如果没有事先添加缓冲液或培养基,则不应直接添加到细胞中。一旦形成水凝胶,不要试图进一步混合凝胶,因为这有可能破坏水凝胶的完整性。操作细胞时应小心谨慎,以免破坏支架。

法律信息

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What volume of H2O should be used for reconstitution of Product A6982, HydroMatrix Peptide Cell Culture Scaffold?

    The package size of the A6982 indicates the volume of H2O to use for reconstitution.  For example, a 1 ml package will require 1 ml of  H2O for reconstitution.

  6. Will Product A6982, HydroMatrix Peptide Cell Culture Scaffold, work will for adherent cells and suspension cells?   

    Yes, the product works with both cell types.

  7. What is the concentration of Product A6982, HydroMatrix Peptide Cell Culture Scaffold, when reconstituted as indicated?

    A 1% (w/v) (10 mg/ml) stock solution is accomplished by the addition of sterile dH20 as indicated.

  8. What concentration of HydroMatrix Peptide Scaffold, Product A6982, should I use for my cells?

    A 1% (w/v) solution results in a stronger gel. A working concentration of 0.5% (w/v) results in a soft gel with weak mechanical strength that is suitable for cell migration and angiogenesis.  Working solution concentrations as low as 0.15-0.25% are suitable for many cell types, including neurons. It is recommended for most applications to start with a 0.25% (w/v) working solution (2.5 mg/ml).

  9. How do I embed or encapsulate cells in HydroMatrix Peptide Scaffold, Product A6982, before polymerization?

    In a solution of dH20, HydroMatrix has a pH of 2.0-2.5; therefore it must not be added directly to cells.  The addition of buffer or media is performed prior to the addition of cells.  If performing cell encapsulation studies, addition of iso-osmotic sucrose will help protect the cells until a physiological pH is obtained.

  10. How does HydroMatrix Peptide Cell Culture Scaffold compare to PuraMatrix from Becton-Dickenson?

    Both products are peptide hydrogels.  There is no similarity between the peptides used to create the matrices.  They are both just classified as self-assembling peptide hydrogels. The sequence for the peptide is considered proprietary.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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