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生物来源
mouse
偶联物
alkaline phosphatase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
BN-34, monoclonal
表单
buffered aqueous glycerol solution
技术
dot blot: 1:10,000 using human IgG and biotin-monoclonal anti-human IgG
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil and biotin-monoclonal anti-human IgG
indirect ELISA: 1:30,000 using human IgG and biotin-monoclonal anti-human IgG
western blot (chemiluminescent): 1:10,000 using human IgG and biotin-monoclonal anti-human IgG
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
该抗体可使用竞争性ELISA识别游离的生物素和生物胞素。通过使用间接ELISA或免疫组织化学染色，该抗体还可识别与各种免疫球蛋白耦合的生物素。特异性是通过使用生物素化山羊抗体与涂覆在多孔板上的人和兔抗原而评估的。
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with biotin-keyhole limpet hemocyanin (KLH).
免疫原
生物素-KLH偶联物
应用
可代替亲和素以降低非特异性结合。
该抗体可被用于扩增技术、ELISA、免疫印迹、原位核酸杂交、流式细胞术、图像分析或共聚焦显微镜。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
Monoclonal Anti-Biotin-Alkaline Phosphatase antibody produced in mouse has been used in:
- blotting (western), Southern and dot blots)
- immunohistology
- in situ nucleic acids hybridization
- enzyme linked immunosorbent assay (ELISA)
- fluorescent activated cell-sorting (FACS)
- electron microscopy
生化/生理作用
Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
The high binding affinity of biotin to egg white or bacteria-derived avidin has been exploited in the design of immunoassays and immunohistologic staining techniques. Conventional immunoassay methods are improved using monoclonal anti-biotin, which enhances the sensitivity of avidin-biotin immunoassays by adding a second layer of enzyme via the biotin specific monoclonal antibody.
外形
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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