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生物来源
bacterial (Bacillus amyloliquefaciens)
质量水平
类型
Type II-A
方案
≥30%
表单
lyophilized powder
比活
≥1,500 units/mg protein (biuret)
分子量
50-55 kDa by SDS-PAGE
技术
SDS-PAGE: suitable
溶解性
H2O: soluble 0.1 mg/mL, clear, colorless
适用性
suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
应用
life science and biopharma
储存温度
−20°C
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一般描述
αα-淀粉酶是一种胞外酶，广泛存在于许多动植物中，也分布于微生物中，例如各种芽孢杆菌(Bacillus species)。
α-淀粉酶（1,4-α-d-葡萄糖水解酶），是一种内切葡聚糖酶，属于糖苷水解酶家族13(GH13)的一员。
应用
α-淀粉酶(芽孢杆菌)已用于：
- 作为唾液组分，用于人工咀嚼；胃肠腔消化实验
- 作为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的标准品，测定α-淀粉酶浓度
- 对乙醇不溶物(AIR)脱淀粉，进行非纤维素神经单糖分析
生化/生理作用
α-淀粉酶可将淀粉降解为麦芽糖等寡糖、葡萄糖和α-极限糊精。它可水解由三个或多个α-(1→4)连接D-葡萄糖单元组成的多糖的α-(1→4)葡聚糖键，无需水解α-(1→6)键。参与葡萄糖生成，对于能量获取至关重要。α-淀粉酶是一种广为人知的工业酶，适用于食品、洗涤、纺织、发酵和制药行业。
特点和优势
85 °C，pH 6.2下处理60分钟，依然保持>98%活性。
- α-淀粉酶（来自地衣芽孢杆菌NCIB 6346）在pH 6.2和85°C条件下60分钟后保留98%以上的活性。
- 其他α-淀粉酶在91°C下存放1小时后，仍然具有100%活性。
单位定义
在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
制备说明
4×结晶
以0.1 mg/mL的浓度溶于水，形成透明无色溶液。
其他说明
本产品仅供研&发使用，不得用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Andrzej T Lulko et al.
Applied and environmental microbiology, 73(16), 5354-5362 (2007-06-26)
Transcriptome analysis was used to investigate the global stress response of the gram-positive bacterium Bacillus subtilis caused by overproduction of the well-secreted AmyQ alpha-amylase from Bacillus amyloliquefaciens. Analyses of the control and overproducing strains were carried out at the end
Immobilized Enzymes on Gold Nanoparticles: From Enhanced Stability to Cleaning of Heritage Textiles.
Francesca Gherardi et al.
ACS applied bio materials, 2(11), 5136-5143 (2019-11-18)
Enzyme-based treatments are used in heritage conservation for the effective removal of glues and other damaging organic layers from the surfaces of historic and artistic works. Despite their potential, however, the application of enzymatic treatments is currently limited because of
Vincent T Calabrese et al.
Biotechnology progress, 32(5), 1271-1275 (2016-10-23)
A number of years ago we reported a two-step inactivation mechanism for α-amylase (enzyme) on the basis of theoretical and experimental studies in aqueous solutions. In the first step the metal (Ca2+ ) ion dissociates reversibly from the enzyme followed
Ajaya K Biswal et al.
Biotechnology for biofuels, 7(1), 11-11 (2014-01-24)
Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked
Applications of high-hydrostatic-pressure processing on microbial enzymes
Liu Y, et al.
Journal of biological and chemical chronicles, 331-371 (2023)
实验方案
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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