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Merck
CN

A6380

α-淀粉酶 来源于芽孢杆菌 属

Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)

别名:

α-淀粉酶, 糖原酶, 1,4-α-D-葡聚糖-葡萄糖水解酶

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关于此项目

化学文摘社编号:
9000-90-2
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
MFCD00081319
EC 号:
3.2.1.1 (BRENDA, IUBMB)

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产品名称

α-淀粉酶 来源于芽孢杆菌 属, Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)

biological source

bacterial (Bacillus amyloliquefaciens)

type

Type II-A

assay

≥30%

form

lyophilized powder

specific activity

≥1,500 units/mg protein (biuret)

mol wt

50-55 kDa by SDS-PAGE

technique(s)

SDS-PAGE: suitable

solubility

H2O: soluble 0.1 mg/mL, clear, colorless

suitability

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

application(s)

life science and biopharma

storage temp.

−20°C

Quality Level

200

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Application

α-淀粉酶(芽孢杆菌)已用于：

  • 作为唾液组分，用于人工咀嚼；胃肠腔消化实验
  • 作为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的标准品，测定α-淀粉酶浓度
  • 对乙醇不溶物(AIR)脱淀粉，进行非纤维素神经单糖分析

Biochem/physiol Actions

α-淀粉酶可将淀粉降解为麦芽糖等寡糖、葡萄糖和α-极限糊精。它可水解由三个或多个α-(1→4)连接D-葡萄糖单元组成的多糖的α-(1→4)葡聚糖键，无需水解α-(1→6)键。参与葡萄糖生成，对于能量获取至关重要。α-淀粉酶是一种广为人知的工业酶，适用于食品、洗涤、纺织、发酵和制药行业。

Features and Benefits

85 °C，pH 6.2下处理60分钟，依然保持>98%活性。
  • α-淀粉酶（来自地衣芽孢杆菌NCIB 6346）在pH 6.2和85°C条件下60分钟后保留98%以上的活性。
  • 其他α-淀粉酶在91°C下存放1小时后，仍然具有100%活性。

General description

αα-淀粉酶是一种胞外酶，广泛存在于许多动植物中，也分布于微生物中，例如各种芽孢杆菌(Bacillus species)。
α-淀粉酶（1,4-α-d-葡萄糖水解酶），是一种内切葡聚糖酶，属于糖苷水解酶家族13(GH13)的一员。

Other Notes

在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer
本产品仅供研&发使用，不得用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。

Preparation Note

4×结晶
以0.1 mg/mL的浓度溶于水，形成透明无色溶液。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000462703
0000462701
0000462701
0000462703
0000458447

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Sun-Li Chong et al.
Analytical and bioanalytical chemistry, 401(9), 2995-3009 (2011-09-10)
The atmospheric pressure matrix-assisted laser desorption/ionization with ion trap mass spectrometry (AP-MALDI-ITMS) was investigated for its ability to analyse plant-derived oligosaccharides. The AP-MALDI-ITMS was able to detect xylooligosaccharides (XOS) with chain length of up to ten xylopyranosyl residues. Though the
Magdalena Eder et al.
Journal of phycology, 44(5), 1221-1234 (2008-10-01)
The cell wall of the green alga Micrasterias denticulata Bréb. ex Ralfs (Desmidiaceae, Zygnematophyceae, Streptophyta) was investigated to obtain information on the composition of component polysaccharides and proteoglycans to allow comparison with higher plants and to understand cell wall functions
Vincent T Calabrese et al.
Biotechnology progress, 32(5), 1271-1275 (2016-10-23)
A number of years ago we reported a two-step inactivation mechanism for α-amylase (enzyme) on the basis of theoretical and experimental studies in aqueous solutions. In the first step the metal (Ca2+ ) ion dissociates reversibly from the enzyme followed
Francesca Gherardi et al.
ACS applied bio materials, 2(11), 5136-5143 (2019-11-18)
Enzyme-based treatments are used in heritage conservation for the effective removal of glues and other damaging organic layers from the surfaces of historic and artistic works. Despite their potential, however, the application of enzymatic treatments is currently limited because of
Andrzej T Lulko et al.
Applied and environmental microbiology, 73(16), 5354-5362 (2007-06-26)
Transcriptome analysis was used to investigate the global stress response of the gram-positive bacterium Bacillus subtilis caused by overproduction of the well-secreted AmyQ alpha-amylase from Bacillus amyloliquefaciens. Analyses of the control and overproducing strains were carried out at the end
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实验方案

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

α-淀粉酶酶促测定 (EC 3.2.1.1)

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

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