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Merck
CN

A6017

Sigma-Aldrich

ω-Aminohexyl–Agarose

saline suspension

别名:

Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
23151817
NACRES:
NA.56

生物来源

plant

质量水平

表单

saline suspension

标记范围

≥5 μmol per mL

技术

affinity chromatography: suitable

基质

cross-linked 4% beaded agarose

基质活化

cyanogen bromide

基质附着

amino of 1,6-diaminohexane

基质隔离区

1 atom

容量

≥5 mg/mL binding capacity (bovine serum albumin)

适用性

suitable for chromatography

储存温度

2-8°C

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一般描述

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

应用

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.

生化/生理作用

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.

外形

Suspension in 0.5 M NaCl containing preservative

其他说明

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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N V Bulushova et al.
Biochemistry. Biokhimiia, 76(2), 202-208 (2011-05-17)
A 67-kDa protein that can specifically bind the activated Cry9A endotoxin under ligand-blotting conditions was purified from midgut epithelium apical membranes of wax moth Galleria mellonella by affinity chromatography. N-Terminal amino acid sequencing enabled identification of this protein as aminopeptidase
Heterogeneity of antibodies reactive with the dominant antigen of Actinobacillus actinomycetemcomitans.
Nakashima K, et al.
Infection and Immunity, 65, 3794-3798 (1997)
Use of stable 6-aminohexyl derivatives for labelling polysaccharides with haptens and for preparing polysaccharide immunoadsorbents.
P L Ey
Journal of immunological methods, 160(1), 135-137 (1993-03-15)
L G Martini et al.
Pharmaceutical research, 12(11), 1786-1790 (1995-11-01)
The purpose of this study was to investigate the influence of hydration characteristics on the in vitro release of 5-fluorouracil from a swellable matrix prepared using a novel triblock copolymer of poly(epsilon-caprolactone) and poly(oxyethylene). Matrices were prepared by dry compression
S Mandjiny et al.
Journal of chromatography, 616(2), 189-195 (1993-07-02)
Histidine, a pseudobiospecific ligand, had been utilized to purify several proteins such as chymosin, acidic protease, carboxypeptidase Y and immunoglobulin G (IgG). A detailed study was undertaken to purify IgG on histidine coupled to aminohexyl Sepharose [A. El-Kak and M.

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