A6017
ω-Aminohexyl–Agarose
saline suspension
别名:
Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose
产品名称
ω-Aminohexyl–Agarose, saline suspension
suitability
suitable for chromatography
biological source
plant
form
saline suspension
extent of labeling
≥5 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino of 1,6-diaminohexane
matrix spacer
1 atom
capacity
≥5 mg/mL binding capacity (bovine serum albumin)
storage temp.
2-8°C
Quality Level
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Application
ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.
Biochem/physiol Actions
ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.
General description
Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Physical form
Suspension in 0.5 M NaCl containing preservative
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
K Nakashima et al.
Infection and immunity, 65(9), 3794-3798 (1997-09-01)
The serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 is reported to be the O antigen of lipopolysaccharide, and the highest titers of serum antibody reactive with A. actinomycetemcomitans in early-onset periodontitis (EOP) patients bind SbAg. These high titers
Julio Blanco et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 2), 352-354 (2002-01-25)
Aspartokinase III catalyzes the commitment step in the aspartate metabolism pathway, the phosphorylation of aspartic acid. The Escherichia coli enzyme has been crystallized in the presence of its natural substrate (aspartic acid) and Mg-ADP and diffraction data has been collected
Y Ruttyn et al.
Journal of chromatography, 491(2), 299-308 (1989-07-21)
Human plasma was chromatographed under different experimental conditions on aminohexyl Sepharose. A strong retention of factor VIII/vWf and of factors VII, II, IX and X was observed. A satisfactory stabilization of eluted factor VIII clotting activity was obtained after addition
Hydrophobic chromatography: use for purification of glycogen synthetase.
Shaltiel S and Er-El Z
Proceedings of the National Academy of Sciences of the USA, 70, 778-781 (1973)
D Couchie et al.
The Biochemical journal, 199(2), 441-446 (1981-11-01)
Chromatography on hexyl-agarose resolved a partially purified cyclic GMP-activated phosphodiesterase from rat liver into two peaks of activity: the first was eluted with 0.5 M-KCl and was cyclic AMP-specific. The second was tightly bound to hexyl-agarose and was not eluted
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