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Merck
CN
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文件

A6014

Sigma-Aldrich

吖啶橙 半(氯化锌) 盐

For nucleic acid staining in cells or gels

别名:

3,6-双(二甲基氨基)吖啶氯化物半(氯化锌盐), 3,6-双(二甲基氨基)吖啶盐酸盐 氯化锌复盐, 碱性橙14

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About This Item

线性分子式:
C17H20ClN3 · HCl · 1/2ZnCl2
CAS号:
10127-02-3
分子量:
369.96
颜色索引号:
46005
Beilstein:
3734978
EC 号:
233-353-6
MDL编号:
MFCD00081043
UNSPSC代码:
12171500
PubChem化学物质编号:
24891048
NACRES:
NA.52

质量水平

200

产品线

BioReagent

形式

powder

组成

Dye content, ~80%

技术

nucleic acid detection: suitable

溶解性

ethanol: 2 mg/mL
 4 mg/mL (2-methoxyethanol (EGME))
water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)

适用性

suitable for flow cytometry
suitable for microscopy

储存温度

room temp

SMILES字符串

Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C

InChI

1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2

InChI key

RAHGLSRJKRXOSY-UHFFFAOYSA-L

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一般描述

吖啶橙是一种异染性荧光阳离子染料，可渗透细胞膜并嵌入DNA和RNA。它能够实现在琼脂糖和聚丙烯酰胺凝胶上目视检测核酸。

应用

吖啶橙是一种细胞可渗透的异染色荧光阳离子染料，可嵌入 DNA 和 RNA，用于荧光和表观显微镜检测。吖啶橙染料已被用于通过流式细胞术分析线粒体和溶酶体含量，表征多药耐药性以及检测大鼠胸腺细胞凋亡过程中线粒体质量的变化。
适合
  • 检测凝胶电泳分离的核酸
  • 荧光和落射荧光显微镜
  • 流式细胞术分析线粒体和溶酶体
  • 凋亡研究中的DNA染色

特点和优势

  • 120 μM吖啶橙在单链和双链多核苷酸凝胶
  • 差异染色的每个条带中可检测到25-50 ng的纯化DNA

原理

吖啶橙嵌入双螺旋核酸中，在530 nm处可检测到绿色荧光。它与单链核酸中的磷酸基团静电结合，在640 nm处可检测到红色荧光。

相关产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Warning

危险声明

H341

危险分类

Muta. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Z Darzynkiewicz et al.
Cytometry, 13(8), 795-808 (1992-01-01)
The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA
G K McMaster et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)
We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The
A B Borisov et al.
Tsitologiia, 24(10), 1233-1237 (1982-10-01)
Using supravital fluorescent staining of lysosomes with Euchrysine 3R, the morphology of these organelles was studied in L cells examined from cultures being at different growth phases in the course of cell cycle and after adipocyte conversion of L cells
Yu Sato et al.
Anatomical science international, 94(2), 199-208 (2019-01-03)
Neurons are classified into several morphological types according to the locations of their somata and the branching patterns of their axons and dendrites. Recent studies suggest that these morphological features are related to their physiological properties, including firing characteristics, responses
H Baisch et al.
Cell proliferation, 32(5), 303-319 (2000-01-05)
Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of
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