一般描述
This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.
特异性
该抗体可识别合成聚组氨酸，以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。
免疫原
重组聚组氨酸标签融合蛋白。
应用
Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
外形
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
制备说明
Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.
法律信息
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Eric A Davidson et al.
Current protocols in molecular biology, Chapter 24, Unit 24-Unit 24 (2009-07-04)
This unit describes a protocol for the directed evolution of proteins utilizing in vitro compartmentalization. This method uses a large number of independent in vitro transcription and translation (IVTT) reactions in water droplets suspended in an oil emulsion to enable
Ryan T Cameron et al.
Molecular and cellular neurosciences, 61, 46-55 (2014-05-27)
Up-regulation of Hsp20 protein levels in response to amyloid fibril formation is considered a key protective response against the onset of Alzheimer's disease (AD). Indeed, the physical interaction between Hsp20 and Aβ is known to prevent Aβ oligomerisation and protects
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