推荐产品
生物来源
mouse
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
1A4, monoclonal
表单
buffered aqueous glycerol solution
分子量
antigen ~42 kDa
种属反应性
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
技术
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
同位素/亚型
IgG2a
UniProt登记号
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Acta2(11475)
rat ... Acta2(81633)
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一般描述
其抗体（也称为抗α-Sm-1）对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应，并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
肌动蛋白是一种高度保守的蛋白质，是所有细胞类型中细胞骨架和收缩结构的主要组成部分。肌动蛋白含量与分化类型以及细胞和组织的功能状态有关。肌动蛋白具有两种不同的聚集形式，即球状或纤维状，并且在脊椎动物中至少具有六种不同亚型。肌动蛋白表现出大于90％的序列同源性，但每种亚型都具有不同的NH 2-末端序列。同工型由三种α肌动蛋白（骨骼肌，心脏，平滑肌），一种β肌动蛋白（β-非肌肉）和两种γ肌动蛋白（γ平滑肌和γ非肌肉）组成。
免疫原
N-末端合成的α-平滑肌肌动蛋白十肽。
应用
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长，并在4％的50％丙酮/乙醇中固定10分钟。
对固定在1%PFA中的角质细胞进行免疫细胞化学，并与小鼠单克隆抗平滑肌肌动蛋白（14A）以1:100的稀释度孵育。
小鼠单克隆抗肌动蛋白，α−平滑肌-碱性磷酸酶抗体可用于小鼠和人体组织的免疫组织化学应用。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫组织化学（1篇论文）
免疫组织化学（1篇论文）
外形
含有1 mM MgCl2、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠（用作防腐剂）的0.05M Tris缓冲液，pH 8.0中的溶液。
其他说明
想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Alex H P Chan et al.
PloS one, 12(3), e0174773-e0174773 (2017-03-30)
Current animal models for the evaluation of synthetic grafts are lacking many of the molecular tools and transgenic studies available to other branches of biology. A mouse model of vascular grafting would allow for the study of molecular mechanisms of
Kenneth Chan et al.
Journal of the American Heart Association, 6(11) (2017-11-02)
Genome-wide association studies identified ADAMTS7 as a risk locus for coronary artery disease (CAD). Functional studies suggest that ADAMTS7 may promote cellular processes in atherosclerosis. We sought to examine the association between genetic variation at ADAMTS7 and measures of atherosclerosis
J V Jester et al.
Investigative ophthalmology & visual science, 40(9), 1959-1967 (1999-08-10)
Recent studies indicate that transforming growth factor (TGF)beta is a potent inducer of corneal myofibroblast differentiation and expression of smooth muscle-specific, alpha-actin (alpha-SMA). Although TGFbeta is known to enhance synthesis of extracellular matrix proteins and receptors, little is known about
Maryellen Sandor et al.
Eplasty, 17, e1-e1 (2017-01-26)
Objective: Benchtop methods were evaluated for preclinical inflammation/capsule formation correlation following implantation of human acellular dermal matrices. Methods: Dermal matrices were compared with native dermis for structure (histology, scanning electron microscopy), collagen solubility (hydroxyproline), enzymatic susceptibility (collagenase), and thermal stability
Izabela Rozenberg et al.
Arteriosclerosis, thrombosis, and vascular biology, 30(5), 923-930 (2010-03-06)
Enhanced endothelial permeability leading to intimal accumulation of low-density lipoproteins (LDL) stimulates the formation of atherosclerotic lesions. Histamine is known to increase vascular permeability. Whether this affects the formation of atherosclerotic lesions, however, remains elusive. Apolipoprotein E-null (ApoE(-/-)) mice treated
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