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生物来源
bovine
质量水平
检测方案
≥96% (agarose gel electrophoresis)
形式
lyophilized powder
分子量
~66 kDa
包装
poly bottle of
来源
USA origin
技术
immunohistochemistry: suitable
pH值（酸碱度）
4.8-7.5
溶解性
H2O: soluble 40 mg/mL, clear to slightly hazy, yellow
UniProt登记号
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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一般描述
Serum albumins are soluble constituents of the circulatory system. Bovine serum albumin (BSA) is homologous to human serum albumin (HSA). BSA consists of three homologous domains (I, II, III), that are separated into nine loops by disulfide bonds. Serum albumin acts as a depot protein.
应用
Bovine Serum Albumin has been used:
- for in vitro high molecular weight (HMW)-species formation assay
- for immunohistochemistry
- in Escherichia coli quantitative polymerase chain reaction (qPCR) analysis
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
制备说明
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
This is a cold alcohol fractionated albumin that is produced by Sigma-Aldrich with a stricter adherence to the original Cohn method of fractionation than other commercially available bovine albumins.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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