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Merck
CN

A5588

Sigma-Aldrich

抗-多组氨酸−碱性磷酸酶抗体,小鼠单克隆

clone HIS-1, purified from hybridoma cell culture

别名:

聚组氨酸单克隆抗体, 单克隆抗-多组氨酸-碱性磷酸酶 小鼠抗, 6 His表位标签单克隆抗体, 6xHis标签单克隆抗体, HHHHHH表位标签单克隆抗体, Hexa His标签单克隆抗体, His6标签单克隆抗体, 组氨酸标签单克隆抗体, 聚组氨酸标签单克隆抗体

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

mouse

质量水平

偶联物

alkaline phosphatase conjugate

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

HIS-1, monoclonal

表单

buffered aqueous glycerol solution

技术

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

同位素/亚型

IgG2a

运输

wet ice

储存温度

2-8°C

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一般描述

单克隆抗-多组氨酸-碱性磷酸酶抗体可识别天然和变性还原形式的合成型多组氨酸或多组氨酸标记的融合蛋白。该产物可与由原核pET、pRSET和pTrc表达载体表达的融合蛋白反应。

特异性

该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。

免疫原

重组聚组氨酸标签融合蛋白。

应用

抗体适用于ELISA、免疫印迹和斑点印迹检测。

外形

含有1 mM MgCl2、1% 牛血清白蛋白、50% 甘油和15 mM 叠氮化钠的 0.05MTris 缓冲液,pH 8.0中的溶液

法律信息

该产品受DE 19507166专利及独家授权给QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany的国外同等专利保护。

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

含少量动物源组分生物产品
常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Application of a Short, Disordered N-Terminal Flagellin Segment, a Fully Functional Flagellar Type III Export Signal, to Expression of Secreted Proteins
Dobo,J et al.
Applied and Environmental Microbiology, 6(3), 891-899 (2010)
Rozbeh Jafari et al.
Microbial cell factories, 10, 34-34 (2011-05-18)
Optimization of conditions during recombinant protein production for improved yield is a major goal for protein scientists. Typically this is achieved by changing single crucial factor settings one at a time while other factors are kept fixed through trial-and-error experimentation.
Min Yang et al.
Nature chemical biology, 14(12), 1109-1117 (2018-11-14)
The elucidation and prediction of how changes in a protein result in altered activities and selectivities remain a major challenge in chemistry. Two hurdles have prevented accurate family-wide models: obtaining (i) diverse datasets and (ii) suitable parameter frameworks that encapsulate
Pooja Bhardwaj et al.
Antimicrobial agents and chemotherapy, 60(4), 2209-2221 (2016-01-27)
Chlorhexidine is a bisbiguanide antiseptic used for infection control. Vancomycin-resistantE. faecium(VREfm) is among the leading causes of hospital-acquired infections. VREfm may be exposed to chlorhexidine at supra- and subinhibitory concentrations as a result of chlorhexidine bathing and chlorhexidine-impregnated central venous
Sarah E Partovi et al.
The Journal of biological chemistry, 293(14), 5236-5246 (2018-02-08)
For nearly 30 years, coenzyme M (CoM) was assumed to be present solely in methanogenic archaea. In the late 1990s, CoM was reported to play a role in bacterial propene metabolism, but no biosynthetic pathway for CoM has yet been

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