生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
4A1/3, monoclonal
形式
buffered aqueous solution
分子量
antigen ~23 kDa
种属反应性
mouse, human
浓度
~1.0 mg/mL
技术
western blot: 2-4 μg/mL using whole extract of human Jurkat or Raji cells
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ASF1A(25842)
一般描述
ASF1 is a histone chaperone protein that binds H3 and H4. It functions in nucleosome assembly.
Monoclonal Anti-ASF1 (mouse IgG2a isotype) is derived from the hybridoma 4A1/3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an ASF1A fusion protein. ASF1 (anti-silencing function 1) encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast.
免疫原
ASF1A fusion protein
应用
Monoclonal Anti-ASF1 antibody produced in mouse has been used in immunoblotting.
生化/生理作用
ASF1 (anti-silencing function 1) protein is a key component of a histone donor complex. It interacts with histones H3 and H4 and functions together with a chromatin assembly factor 1 (CAF-1) during DNA replication and repair. ASF1 also contributes to genome stability during S phase. Cells lacking ASF1 were found to be sensitive to a number of genotoxic agents that predominantly cause DNA damage by interfering with DNA replication fork progression such as hydroxyurea and camptothecin.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis
Lario LD, et al.
Plant Physiology, 162(2), 1164-1177 (2013)
Differential requirements for Tousled-like kinases 1 and 2 in mammalian development
Segura-BS, et al.
Cell Death and Differentiation, 24(11), 1872-1872 (2017)
Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C
Franco AA, et al.
Genes & Development, 19(11), 1365-1375 (2005)
Sandra Segura-Bayona et al.
Cell death and differentiation, 24(11), 1872-1885 (2017-07-15)
The regulation of chromatin structure is critical for a wide range of essential cellular processes. The Tousled-like kinases, TLK1 and TLK2, regulate ASF1, a histone H3/H4 chaperone, and likely other substrates, and their activity has been implicated in transcription, DNA
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