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Merck
CN
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文件

A50120

Sigma-Aldrich

DEAE-葡聚糖凝胶®

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别名:
交葡聚糖凝胶®
CAS号:
39455-31-7
MDL编号:
MFCD00132778
UNSPSC代码:
47101511
NACRES:
NA.56

形式

powder

质量水平

200

技术

protein purification: suitable

基质

cross-linked dextran

珠子尺寸

40-125 μm (dry)

孔径

~200,000 Da exclusion limit

pH值（酸碱度）

2—12

容量

3-4 meq/g binding capacity

相关类别

一般描述

A50120-100G的更新产品编号为 GE17-0180-01
A50120-500G的更新产品编号为 GE17-0180-02

应用

DEAE葡聚糖凝胶可用于纯化和分离蛋白质的离子交换色谱。它已被用于鉴别和标记急性心肌梗死患者（AMI）体内胰岛素激活的一氧化氮合酶抑制剂（蛋白质）。

法律信息

Sephadex is a registered trademark of Cytiva

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the procedure for swelling Product A50120, DEAE-Sephadex® resin?

    Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.

  4. What procedures are used for elution when using Product A50120, DEAE-Sephadex® resin?

    For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.

  5. How can Product A50120, DEAE-Sephadex® resin be regenerated?

    Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.

  6. How can Product A50120, DEAE-Sephadex® resin, be stored?

    Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

E Okuno et al.
Biochimica et biophysica acta, 841(1), 112-119 (1985-07-26)
Quinolinic acid phosphoribosyltransferase (EC 2.4.2.19) was purified 3600-fold from rat liver and 280-fold from rat brain. Kinetic analyses (Km = 12 microM for the substrate quinolinic acid and Km = 23 microM for the cosubstrate phosphoribosylpyrophosphate), physicochemical properties of the
Misook Kim et al.
Biochimica et biophysica acta, 1770(12), 1627-1635 (2007-10-09)
Two cysteine proteases, GP2 and GP3, have been isolated from ginger rhizomes (Zingiber officinale). GP2 is virtually identical to a previously identified ginger protease GPII [K.H. Choi, and R.A. Laursen, Amino-acid sequence and glycan structures of cysteine proteases with proline
M A Zaval'nyĭ et al.
Voprosy virusologii, (5)(5), 583-589 (1980-09-01)
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead
P K Farmer et al.
Biochimica et biophysica acta, 1434(1), 6-17 (1999-11-11)
A calmodulin-like domain protein kinase (DcCPK1, previously designated CDPK431) cloned from carrot (Daucus carota L.) was expressed at high levels in Escherichia coli and partially purified. Ca(2+)-induced gel mobility shift and (45)Ca(2+) ligand binding assays confirmed that recombinant DcCPK1 binds
Clovis R Nakaie et al.
Analytical biochemistry, 318(1), 39-46 (2003-06-05)
This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports can be used for peptide synthesis. Moreover, due to the high stability of
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