推荐产品
生物来源
goat
质量水平
偶联物
peroxidase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
direct ELISA: 1:2,000
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
正在寻找类似产品? 访问 产品对比指南
一般描述
生物素是活生物体和培养物中细胞所需的必需维生素。生物素与蛋白亲和素或细菌来源的链霉亲和素的高结合亲和力已被用于设计用于检测抗原与抗体的相互作用的免疫测定法。山羊抗生物素过氧化物酶抗体可用于许多可能将生物素引入靶标的应用。
通过 Ouchterlony 双扩散和免疫电泳与生物素载体蛋白复合物测定特异性和反应性。未观察到与载体蛋白的反应性。
免疫原
生物素-KLH 结合物
应用
使用 HRP 标记的山羊抗生物素抗体，通过蛋白质印迹分析 U937 和 BHK 细胞裂解物中的生物素标记蛋白。
在一些应用中，生物素化探针与亲和素的定位会产生高背景水平。抗生物素试剂可替代亲和素，以减少非特异性结合。
山羊抗生物素过氧化物酶抗体可用于直接 ELISA 分析（稀释度为 1：2,000）。
外形
0.01 M磷酸盐缓冲液，pH 7.4，含有1％牛血清白蛋白和防腐剂。
制备说明
通过 Wilson,M.B. 和 Nakane,P.K. 在《免疫荧光及相关染色技术》(Immunofluorescence and Related Staining Techniques) 中描述的过碘酸盐法制备（Elsevier/North Holland Biomedical Press 出版社，Amsterdam,p215 (1978)）。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
未找到合适的产品？
试试我们的产品选型工具.
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
Katarzyna Kwiatkowska et al.
Journal of cell science, 116(Pt 3), 537-550 (2003-01-01)
Activation of Fcgamma receptor II (FcgammaRII) induces rearrangement of the actin-based cytoskeleton that serves as a driving force for FcgammaRII-mediated phagocytosis and FcgammaRII capping. To get insight into the signaling events that lead to the actin reorganization we investigated the
Soledad Astrada et al.
Journal of peptide science : an official publication of the European Peptide Society, 22(11-12), 711-722 (2016-12-10)
Because of resistance development by cancer cells against current anticancer drugs, there is a considerable interest in developing novel antitumor agents. We have previously demonstrated that CIGB-552, a novel cell-penetrating synthetic peptide, was effective in reducing tumor size and increasing
Samuel H Henager et al.
Nature methods, 13(11), 925-927 (2016-11-01)
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-terminal cysteine (N-Cys)-containing peptides, but the requirement of a Cys residue at the ligation junction can limit the utility of
Nataliya Gorinski et al.
The Journal of biological chemistry, 295(18), 5970-5983 (2020-03-19)
Barttin is the accessory subunit of the human ClC-K chloride channels, which are expressed in both the kidney and inner ear. Barttin promotes trafficking of the complex it forms with ClC-K to the plasma membrane and is involved in activating
Ee Lin Wong et al.
Nature communications, 10(1), 66-66 (2019-01-10)
Protein-templated fragment ligations have been established as a powerful method for the assembly and detection of optimized protein ligands. Initially developed for reversible ligations, the method has been expanded to irreversible reactions enabling the formation of super-additive fragment combinations. Here
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门