一般描述
eAMV™ 逆转录酶是一种增强型禽成髓细胞病病毒（AMV）逆转录酶，负责合成与 RNA、DNA 或 RNA:DNA 杂交体互补的 DNA 链。这种异常强大的 AMV逆转录酶比标准 AMV 或 Moloney 鼠白血病病毒（M-MLV）逆转录酶具有更优的热稳定性。eAMV™ RT 是从总 RNA 或 poly（A）+RNA获得高质量全长 cDNA 的理想酶，并且在转录长靶标方面也是有效的。
应用
Enhanced Avian Reverse Transcriptase可将RNA转录为DNA，同时显著提升mRNA模板合成cDNA的效率。这是由于本款增强型AMV-RT能够逆转录长链mRNA模板，能够有效穿越复杂的二级结构，可以通过RT-PCR反应检测低丰度mRNA。
增强型禽逆转录酶[eAMV™逆转录酶]已用于总 RNA 的逆转录，以在定量逆转录 PCR（RT-qPCR）分析中合成 cDNA。
生化/生理作用
逆转录酶能够加速dNTP形成磷酸二酯键，从而催化RNA模板整合到互补DNA中。
特点和优势
- 对低丰度信使核糖核酸的敏感性更高
- 在高温（高达 65°C）下通过复杂二级结构进行的无核转录
- 高效生成高达 14.1kb的全长 cDNA
- 产生可用于 PCR 扩增的第一链 cDNA
包装
提供1管10x反应缓冲液。
单位定义
一单位酶能够以多聚腺苷酸为模板，以12-18的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA沉淀材料中。
法律信息
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 5-5 (1989)
商品
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
相关内容
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
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