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等级
for molecular biology
质量水平
检测方案
≥99.0%
形式
powder
技术
affinity binding assay: suitable
protein purification: suitable
杂质
≤0.005% Insolubles
pH值（酸碱度）
5.0-6 (25 °C, 132 g/L)
mp
>280 °C (dec.) (lit.)
溶解性
H2O: 0.667 g/mL
密度
1.77 g/mL at 25 °C (lit.)
痕量阴离子
chloride (Cl-): ≤5 ppm
痕量阳离子
Cu: ≤5 ppm
Fe: ≤5 ppm
Zn: ≤5 ppm
heavy metals (as Pb): ≤2 ppm
吸光度
≤0.025 at 280 at 1 M
≤0.03 at 260 at 1 M
适用性
suitable for flow cytometry
suitable for molecular biology
应用
metabolomics
microbiology
异质活性
DNase, RNase, and protease, none detected
SMILES字符串
N.N.OS(O)(=O)=O
InChI
1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
InChI key
BFNBIHQBYMNNAN-UHFFFAOYSA-N
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一般描述
硫酸铵是一种用途广泛的无机硫酸盐，在生化研究、分子生物学和细胞培养中具有至关重要的作用。它是纯化抗体和沉淀或分离蛋白质不可或缺的工具。此外，它有助于核酸和蛋白质的晶体学分析，还可用作合成实验室培养基中的氮补充剂。 我们的精品硫酸铵经过精心配制，不含 DNase、RNase 和蛋白酶，确保其适合尖端研究。
应用
硫酸铵有助于细胞蛋白的快速沉淀。 硫酸铵可用于蛋白质的沉淀或分级，或抗体的纯化。它也用于核酸和蛋白质的晶体学分析。硫酸铵已用于
- 蛋白的′蓝银′ 胶体考马斯染液的制备。
- 沉淀培养上清液中的志贺样毒素（Shiga-like toxin）。
- 沉淀培养上清液中的病毒颗粒。
特点和优势
- 重金属（以铅计）：≤2 ppm
- 不含 RNase/DNase和蛋白酶。
其他说明
For additional information on our range of Biochemicals, please complete this form.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry
Wayan Suardana et al.
Asian Pacific Journal of Cancer Prevention, 23-10, 3247-3252 (2022)
Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization
David S Powlson, Chris J Dawson
Soil use and management, 38(1), 622-634 (2022)
Protein precipitation using ammonium sulfate
P Wingfield
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 3 (2001)
William P Heal et al.
Nature protocols, 7(1), 105-117 (2011-12-24)
A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme
Mohd Baasir Gaisawat et al.
Microorganisms, 8(1) (2020-01-08)
Clostridium (C.) difficile-infection (CDI), a nosocomial gastrointestinal disorder, is of growing concern due to its rapid rise in recent years. Antibiotic therapy of CDI is associated with disrupted metabolic function and altered gut microbiota. The use of probiotics as an
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