跳转至内容
Merck
CN
所有图片(2)

文件

安全信息

A4268

Sigma-Aldrich

α-淀粉酶 来源于猪胰腺

greener alternative

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

登录查看公司和协议定价
所有图片(2)
别名:
PPA, al1,4 葡聚糖-4-葡萄糖水解酶，, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶
EC 号:
3.2.1.1 (BRENDA, IUBMB)
MDL编号:
MFCD00081319
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物来源

Porcine pancreas

质量水平

300

类型

Type I-A

形式

saline suspension

比活

700-1400 units/mg protein (E1%/280)

分子量

51-54 kDa

环保替代产品特性

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

activity assay: suitable

适用性

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

应用

diagnostic assay manufacturing

环保替代产品分类

, Enabling

储存温度

2-8°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

分子质量：51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅《Biofiles》的文章。

应用

α-淀粉酶用于水解α-连接的多糖α键，如淀粉和糖原。Sigma 公司生产的α-淀粉酶已被用于各种植物研究，如拟南芥代谢研究。

生化/生理作用

α-淀粉酶可水解三个或更多 α-(1,4)连接的 D-葡萄糖单位多糖中的 α-(1,4)葡聚糖键。天然底物，如淀粉和糖原，被分解成葡萄糖和麦芽糖。来自猪胰腺的α-淀粉酶是一种糖蛋白，由约 475 个残基的单一多肽链组成，含有 2 个 SH 基团和 4 个二硫键以及保持稳定性所必需的紧密结合的 Ca2+。

单位定义

在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。

外形

悬浮于含 3 mM CaCl2 的 2.9 M NaCl 溶液中。

制备说明

2 次结晶

其他说明

有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

底物

产品编号
说明
价格

抑制剂

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P342 + P311

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Doudou Huang et al.
Drug design, development and therapy, 15, 5001-5010 (2021-12-25)
Diabetes is a common disease caused by a combination of genetic and environmental factors, which was the top three diseases threatening human health. Therefore, it is necessary to seek more efficient hypoglycemic drugs. The main objective of this study was
Lijiao Kan et al.
Food chemistry, 361, 130047-130047 (2021-05-25)
Inhibition of maltase, sucrase, isomaltase and glucoamylase activity by acarbose, epigallocatechin gallate, epicatechin gallate and four polyphenol-rich tea extract from white, green, oolong, black tea, were investigated by using rat intestinal enzymes and human Caco-2 cells. Regarding rat intestinal enzyme
Saber Abdelkader Saidi et al.
Heliyon, 8(12), e11954-e11954 (2022-12-09)
The study evaluated the phytochemical composition of Ephedra alata and its effects on α-amylase and lipase enzymes and diabetic-induced liver-kidney-testes toxicities to determine the anti-diabetic, anti-obesity, and anti-toxic potentials of the plant. Obesity was induced by a high-fat and fructose
Annabel Bijttebier et al.
Carbohydrate research, 345(2), 235-242 (2009-12-08)
Amylopectin fine structures were studied following limited hydrolysis of gelatinised waxy maize starch by amylases with a different level of inner chain attack (LICA). This was done by size exclusion chromatography as well as by debranching the (partially hydrolysed) amylopectin
Bjarte Aarmo Lund et al.
Molecules (Basel, Switzerland), 26(23) (2021-12-11)
The determination of the temperature dependence of enzyme catalysis has traditionally been a labourious undertaking. We have developed a new approach to the classical Arrhenius parameter estimation by fitting the change in velocity under a gradual change in temperature. The
查看所有相关文献

实验方案

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

α-淀粉酶酶促测定 (EC 3.2.1.1)

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门