推荐产品
生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~140/90 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using lysates of HeLa cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 0.5-1.0 μg/mL using whole extracts of human HeLa cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PALLD(23022)
一般描述
Palladin exists in humans as 7 different isoforms and is a component of actin-containing microfilaments.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer.
Rabbit anti-Palladin antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide.
Rabbit anti-Palladin antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide.
Palladin is a phosphoprotein, which is widely expressed in vertebrate cells and tissues. It is expressed as three major isoforms of 90, 140, and 200 kDa. Palladin consists of a proline-rich region in the NH2-terminal half of the protein and three tandem Ig C2 domains in the COOH-terminal.
应用
Rabbit anti-palladin antibody can be used for immunoprecipitation (5-10μg), indirect immunofluorescence (2-5μg/mL) and western blot (0.5-1.0μg/mL) using HeLa cells.
生化/生理作用
Palladin controls cell shape, adhesion, and contraction. It interacts with the actin-associated proteins α-actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. The Ig3 domain of palladin binds to F-actin. Its expression is required for normal mammalian embryogenesis since its inactivation leads to embryonic lethality.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Palladin is an actin cross-linking protein that uses immunoglobulin-like domains to bind filamentous actin
Dixon RDS, et al.
Test, 283(10), 6222-6231 (2008)
Identification of palladin isoforms and characterization of an isoform-specific interaction between Lasp-1 and palladin
Rachlin AS and Otey CA
Journal of Cell Science, 119(6), 995-1004 (2006)
Joshua S McLane et al.
Cancer microenvironment : official journal of the International Cancer Microenvironment Society, 9(2-3), 127-139 (2016-09-23)
Several changes have been described in the stroma surrounding a tumor, including changes in cellular composition, altered extracellular matrix composition and organization, and increases in stiffness. Tumor cells are influenced by the composition, organization, and mechanical properties of the microenvironment
Characterization of human palladin, a microfilament-associated protein
Mykka?nen OM, et al.
Molecular Biology of the Cell, 12(10), 3060-3073 (2001)
Kay L Pogue-Geile et al.
PLoS medicine, 3(12), e516-e516 (2006-12-30)
Pancreatic cancer is a deadly disease. Discovery of the mutated genes that cause the inherited form(s) of the disease may shed light on the mechanism(s) of oncogenesis. Previously we isolated a susceptibility locus for familial pancreatic cancer to chromosome location
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门