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安全信息

A3809

Sigma-Aldrich

天门冬酰胺酶 来源于大肠杆菌

lyophilized powder, 100-300 units/mg protein (biuret)

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别名:
L -天冬酰胺酰胺水解酶
CAS号:
9015-68-3
EC 号:
3.5.1.1 (BRENDA, IUBMB)
EC 号:
232-765-3
MDL编号:
MFCD00130565
UNSPSC代码:
12352204
NACRES:
NA.54

形式

lyophilized powder

质量水平

200

比活

100-300 units/mg protein (biuret)

组成

Protein, ≥60%

储存温度

2-8°C

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一般描述

天冬酰胺酶是一种细菌酶，也是一种化疗药物。

应用

天冬酰胺酶（来自大肠杆菌）已用于：
  • 比较从Streptomyces brollosae NEAE-115中纯化的L-天冬酰胺酶的细胞毒性作用
  • 作为天冬酰胺酶检测的标准品，定量分析各种鳗鱼组织中的天冬酰胺酶
  • 引起氨基酸缺乏

生化/生理作用

天冬酰胺酶(ASNase)产品通常从大肠杆菌和菊欧文氏菌中获得。这种酶可抑制肿瘤细胞中的蛋白合成。它在细胞周期的G1期具有高活性。会诱发白血病患者发生胰腺炎。
天冬酰胺酶将天冬酰胺转化为天冬氨酸，可用于酶检测。 天冬酰胺酶可减少淀粉食品中的丙烯酰胺形成。 它还可用作急性淋巴细胞白血病的化疗药物。 A3809产品来自于大肠杆菌，以含有氯化钠的冻干粉末形式提供。
门冬酰胺酶可能通过将必需的左旋门冬酰胺转化为门冬氨酸和氨而导致白血病细胞死亡 。天冬酰胺酶是变构调节酶，对正常细胞功能至关重要。

单位定义

一个单位将在pH8.6，37°C下每分钟从L-天冬酰胺中释放出1.0μmol的氨。

外形

含有氯化钠的冻干粉末

制备说明

色谱纯化产品

分析说明

双缩脲法测定蛋白质。

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Warning

危险声明

H317,H361

危险分类

Repr. 2 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. How should this enzyme (Product No. A3809) be reconstituted?

    Prior to determining the activity of this enzyme, Sigma reconstitutes it directly in deionized water. Solutions should be prepared fresh, as the stability of stock solutions has not been evaluated.

  7. What is the source of this enzyme (Product No. A3809)?

    This enzyme is obtained from a natural extraction of E. coli. It is not from a recombinant source.

  8. What is the molecular weight of this enzyme (Product No. A3809)?

    This enzyme consists of a tetramer composed of four identical subunits of 34,080 Daltons each. The molecular weight of the tetramer is 136,320 Daltons.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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