推荐产品
生物来源
bovine
质量水平
方案
≥96% (agarose gel electrophoresis)
表单
lyophilized powder
分子量
~66 kDa
纯化方式
heat shock fractionation
包装
poly bottle of
来源
New Zealand origin
技术
immunoprecipitation (IP): suitable
pH值(酸碱度)
7
~7 (7 % (w/v) in H2O)
溶解性
water: slightly soluble
UniProt登记号
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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一般描述
Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. It belongs to the serum albumins family. It has 17-disulfide bonds. Albumin is the most abundant plasma protein in humans.
应用
Bovine Serum Albumin has been used:
- as a component in chromatin immunoprecipitation (ChIP IP) buffer
- in combination with electrospun polystyrene (ESPS) fiber mats for fluorescence microscopy
- as a component of blocking solution and immobilzation studies
- as a component in phosphate buffer saline (PBS)
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. High levels of albumin is associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia.
制备说明
来源于新西兰产地的牛血清
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
从最新的版本中选择一种:
分析证书(COA)
Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection
Sensing and Bio-Sensing Research, 100304-100304 (2019)
Micro-chromatin Immunoprecipation (mu ChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Bio-protocol, 6(12), 18-23 (2016)
An Interleukin-23-Interleukin-22 Axis Regulates Intestinal Microbial Homeostasis to Protect from Diet-Induced Atherosclerosis
Immunity, 49(5), 943-957 (2018)
Structural changes during the unfolding of Bovine serum albumin in the presence of urea: A small-angle neutron scattering study
Pramana, 63(2), 363-368 (2004)
Bovine serum albumin interactions with metal complexes
Clujul Medical (1957), 87(4), 215-215 (2014)
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