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安全信息

A3156

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS号:
9048-46-8
EC 号:
232-936-2
MDL编号:
MFCD00130384
UNSPSC代码:
12352202
NACRES:
NA.75

生物来源

bovine

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

检测方案

≥99% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

~66 kDa

纯化方式

cold ethanol and heatshock fractionation

来源

USA origin

技术

cell culture | mammalian: suitable

杂质

≤10% water (Karl Fischer)

pH值（酸碱度）

4.8-7.5

溶解性

water: soluble (5g/50ml)

UniProt登记号

P02769

运输

ambient

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

相关类别

一般描述

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. Albumin is the most abundant plasma protein in humans.

应用

Bovine serum albumin has been used:
  • to prevent sticking of the chromosome to the glass micropipette for elasticity measurements using aspiration
  • to block lung homogenates in the plate for mucin protein enzyme-linked immunosorbent assay
  • in alginate solution as a protein model for drug release kinetics investigation

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. Albumin turnover is seen in infants with iron deficiency anemia. BSA acts as a vital constituent in the cell culture media and favors human embryonic stem cells (hESC) differentiation.
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克和乙醇分级分离相结合的方法进行纯化

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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Acrolein-Activated Matrix Metalloproteinase 9 Contributes to Persistent Mucin Production
Deshmukh HS, et al.
American Journal of Respiratory Cell and Molecular Biology (2012)
Elasticity and Structure of Eukaryote Chromosomes Studied by Micromanipulation and Micropipette Aspiration
Houchmandzadeh B, et al.
The Journal of cell biology, 139(1), 1-12 (1997)
Combination of polymer technology and carbon nanotube array for the development of an effective drug delivery system at cellular level
Riggio C, et al.
Nanoscale Research Letters, 4(7), 668-668 (2009)
Albumin and mammalian cell culture: implications for biotechnology applications
Francis GL
Cytotechnology, 62(1), 1-16 (2010)
Bovine serum albumin interactions with metal complexes
Topalua T, et al.
Clujul Medical (1957), 87(4), 215-215 (2014)
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