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生物来源
avidin from egg white
enzyme from horseradish
质量水平
偶联物
peroxidase conjugate
形式
lyophilized powder
组成
Protein, ≥70% E1%/280
标记范围
0.7-2.0 mol peroxidase per mol avidin
技术
direct ELISA: 1:50,000
溶解性
H2O: soluble 1 mg/mL
PBS: soluble
储存温度
−20°C
一般描述
亲和素是来自于蛋清的同源四聚体蛋白（68kDa）。这种蛋蛋白质与生物素高度结合。因此，亲和素生物素结合已被用于免疫检测，以检测组织中的抗原定位。亲和素-生物素免疫检测的使用增强了该技术的灵敏度并改善了对低含量抗原的检测。
应用
为了检测小鼠血清中的Ga1缺陷型IgA1抗体，可在96孔板中使用血清进行ELISA检测，其中，血清与生物素化HAA（结合IgA1）一起孵育。然后将HRP偶联的亲和素与血清在37℃孵育40分钟。
亲和素-过氧化物酶已被用于检测抗5-溴脱氧尿苷（抗BrdU）结合和OVA-IgG1。
外形
含有柠檬酸盐缓冲液的冻干粉末。
制备说明
Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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