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安全信息

A3059

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
9048-46-8
EC 号:
232-936-2
MDL编号:
MFCD00130384
UNSPSC代码:
12352202
NACRES:
NA.27

生物来源

bovine

质量水平

300

检测方案

≥98%

形式

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

poly bottle of

来源

USA origin

技术

ELISA: suitable
western blot: suitable

杂质

essentially globulin free
 protease, none detected

缺失

≤5%

pH值（酸碱度）

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

P02769

异质活性

BT Virus, none detected
VSV Virus, none detected

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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相关类别

一般描述

牛血清白蛋白（BSA）在血液胶体渗透压的调节中起重要作用。 BSA 是牛血清中的一种主要蛋白，包含583个氨基酸，计算分子量为66,430道尔顿。BSA被用于许多不同的应用和领域，例如：
  • 蛋白质定量（作为标准品）
  • 免疫化学（作为封闭剂）
  • 细胞培养（作为营养物质）

这种特殊的BSA经过专门评估和测试，具有非常低的蛋白酶含量/无蛋白酶状态。 不含蛋白酶的BSA可用于以下应用中：
  • 酶测定
  • 蛋白质测定
  • 蛋白酶敏感型技术，包括：
a）酶免疫法（EIA）
b）核酸杂交
c）放射免疫测定（RIA）

应用

牛血清白蛋白已用于发光分析。

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特点和优势

  • 热休克分级
  • 不含蛋白酶
  • 几乎不含球蛋白

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Is Product A3059, Albumin from bovine serum (BSA), suitable for use in ELISA?

    We have not tested A3059 for specific use in ELISA. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in ELISA, by reducing a potential source of background.

  2. Is Product A3059, Albumin from bovine serum (BSA), suitable for use as a blocking agent in immunoblotting?

    We have not tested A3059 for specific use in immunoblotting as a blocking agent, in quality control. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in blotting experiments, by reducing a potential source of background.  The Technical Bulletin states that "Globulin free albumins are suitable for use in applications where no other proteins should be present".

  3. What is the fatty acid content of Product A3059, Albumin from bovine serum (BSA)?  Can it be considered fatty acid free?

    The fatty acid content of A3059 is not tested in quality control, and it is not reported on our Certificate of Analysis. We would not refer to this product as "fatty acid free".

  4. Is Product A3059, Albumin from bovine serum (BSA), endotoxin-free?

    We have not tested A3059 for endotoxin content. Endotoxin-tested BSA products would include A4919, A9306, A9430, A2934, A9306, A9543, or A3675.

  5. Is Product A3059, Albumin from bovine serum (BSA), acetylated to inactivate nucleases? 

    A3059 is not acetylated. We do not test for the presence of nucleases. Acetylated BSA products would be B2518 or B8894.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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